High-titer secondary antibodies, conjugated to ALP can be combined with Agrisera TMB developing reagent
CCD cameras are often used for visualizing the binding of an antibody to a target protein, through chemiluminescent or fluorescent detection. In cases where a CCD camera is not available in the laboratory, secondary antibodies conjugated to alkaline phosphatase (ALP) can be used, and the reaction can instead be visualized using Agrisera TMB reagents. This way, medium and high abundancy proteins can be visualized easily, and the obtained results can be saved in the lab book (although please remember that there is a fading effect). If you are not certain which secondary antibody to choose, our technical team will promptly answer all your questions. | ![]() |