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Anti-NodGS | Nodulin / glutamate-ammonia ligase-like protein
AS15 3030 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
- Product Info
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Immunogen: KLH-conjugated synthetic peptide derived from a C-terminal of Arabidopsis thaliana NodGS, UniProt: Q8W473 , TAIR: AT3G53180
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunoprecipitation (IP), Immunofluorescence (IF), Western blot (WB) Recommended dilution: 1 : 300-1 :400 (IP), 1 : 3000 (IF), 1 : 2000 (WB) Expected | apparent MW: 94 | 90 kDa
- Reactivity
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Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Medicago truncatula, Theobroma cacao
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Application example
4,5 of total protein (supernatant after 1h at 25.000 xg) from Arabidopsis thaliana suspension culture extracted with 50mM HEPES pH 7,5/75mM NaCl/1mM EGTA/1mM MgCl2/1mM NaF in the presence of protease inhibitors were separated on 8 % SDS-PAGE and blotted 1,5h to a nitrocellulose membrane using tank transfer. Blots were blocked with 5% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500, 1:1000, 1:2.000, and 1:5000 overnight at 4°C without agitation. The antibody solution was decanted and the blot was rinsed, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in 5% milk for 30 min at RT with agitation. The blot was washed as above and developed for 1 min with chemiluminescent detection reagent. Exposure time was 15 seconds.
Courtesy of Dr. Jana Chumová, Laboratoř funkční cytologie, Czech Republic - Background
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Background: NodGS (Nodulin/glutamate-ammonia ligase-like protein) shares homology with nodulins and self-assembles into oligomers which are present in response to flagellin treatment. It contains a C-terminal domain of a prokaryotic glutamine synthetase type I. Protein is mainly localized to cytoplasm and is present in a oligomeric form of ca. 700 kDa.
- Product Citations
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Selected references: Doskocilova et al. (2011). A nodulin/glutamine synthetase-like fusion protein is implicated in the regulation of root morphogenesis and in signalling triggered by flagellin. Planta 234, 459–476. doi: 10.1007/s00425-011-1419-7
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts - Reviews:
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Accessories
AS05 068 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Lupinus luteus
AS08 296 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, N. tabacum, O. sativa, P. sativum, S. oleracea
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)