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Photosynthesis
PSI (Photosystem I)

PSI (Photosystem I)

PSI/PSII ratio can be estimated once a quantitative immunoblot for PsaC (AS10 939) and for either PsbA (AS05 084) or PsbD (AS06 146), is performed in 3-4 replicates. Ratio calculation is done on a sample by sample basis. These ratios are based on the subunits present, whether they are in the assembled photosystem or not. Under some circumstances (eg. high light), there are biological reasons that the subunit numbers do not necessarily match the functional photosystems.

Literature
Brown et al. (2008). Flux capacities and acclimation costs in Trichodesmium from the Gulf of Mexico. Marine Biology 154 (3): 413-422.
Levitan et al. (2010). Regulation of nitrogen metabolism in the marine diazotroph Trichodesmium IMS101 under varying temperatures and atmospheric CO2 concentrations. Environmental Microbiology 12(7): 1899-1912.
Thamatrakoln et al. (2013). A ‘Death-Specific Protein’ in a marine diatom regulates photosynthetic responses to iron and light availability. Proceedings of the National Academy of Science 110(50): 20123-20128 Li G,
Li et al. (2014). The Nitrogen Costs of Photosynthesis in a Diatom under Current and Future pCO2. New Phytologist, 205:533-543.

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Confirmed reactivity

Algae Arabidopsis thaliana Begonia sp. Brassica napus Brassica rapa Bryopsis corticulans Chlamydomonas reinhardtii Chlamydomonas sp. ICE-MDV Chlamydomonas sp. UWO241 Chlorella sorokiniana Chlorella vulgaris Chromochloris zofingiensis Colobanthus quitensis Craterostigma pumilum Cyanobacteria Cyanophora paradoxa Cytisus cantabricus (Wilk.) Rchb. F. Dactylis glomerata Dianthus caryophyllus Diatoms Dinoflagellates Dioxoniella giordanoi Drosera capensis Echinochloa crus-galli Emiliania huxleyi Euglena gracilis Euonymus japonicus Flaveria sp. Fraxinus ornus Fucus vesiculosus Gonyaulax polyedra Haematococcus pluvialis Halomicronema hongdechloris Heterosigma akashiwo Hieracium pilosella Higher plants Hordeum vulgare Lactuca sativa Lasallia hispanica Lichens Lolium perenne Marchantia polymorpha Mesembryanthemum sp. Mesostigma viride Micromonas pusilla Mosses Nannochloropsis gaditana Nannochloropsis oceanica IMET1 Neochloris oleoabundans Nicotiana benthamiana Nicotiana tabacum Oryza sativa Phaeodactylum tricornutum Phaseolus vulgaris Physcomitrium patens Picea abies Picea glauca Pinus strobus Pinus sylvestris Pisum sativum Porphyra sp. Scenedesmus obliquus Setaria viridis Sinapsis alba Solanum lycopersicum Spinacia oleracea Stylophora pistillata Synechococcus sp. PCC 7002 Synechococcus sp. PCC 7942 Synechocystis sp. PCC 6803 Syntrichia muralis (Hedw.) Raab Thalassiosira pseudonana Thalassiosira punctigera Tillandsia flabellate Trichodesmium erythraeum Triticale Triticum aestivum Turbinaria reniformis Ulva prolifera Zea mays

Predicted reactivity

Algae Aloysia triphylla Arabidopsis thaliana Arachis hypogaea Avena sativa Beta vulgaris Bigelowiella natans Borago officinalis Brachypodium distachyon Brassica napus Brassica rapa Cannabis sativa Catalpa bungei Cercidiphyllum japonicum Chlamydomonas reinhardtii Chlorella sp. Chlorella vulgaris Chlorokybus atmophyticus Chromera velia Citrus x limon Conifers Cucumis melo Cyanidioschyzon merolae Cyanidioschyzon merolae 10D Cyanobacteria Dicots Exbucklandia populnea Geranium endressii Glycine max Glycine soja Gunnera manicata Indocalamus sinicus Kalanchoe laciniata Lagenaria siceraria Lippia alba Lippia origanoides Lotus japonicus Manihot esculenta Marchantia polymorpha Medicago truncatula Micromonas sp. Monocots Monsonia emarginata Morus notabilis Mytilaria laosensis Nannochloropsis sp. Nicotiana benthamiana Nicotiana sylvestris Nicotiana tabacum Oryza sativa Ostreococcus tauri Panax ginseng Pandanus utilis Parnassia laxmannii Pelargonium cotyledonis Pennisetum americanum Phaeodactylum tricornutum Phaseolus lunatus Phaseolus pachyrrhizoides Phaseolus vulgaris Phyla dulcis Physcomitrium patens Picea spinulosa Pinus thunbergii Populus alba Populus balsamifera Populus canadensis Populus trichocarpa Prochlorococcus sp. Rhodoleia championii Rhyticaryum macrocarpum Ribes fasciculatum Ricinus communis Salvia miltiorrhiza Setaria italica Solanum lycopersicum Solanum tuberosum Spinacia oleracea Synechococcus sp. PCC 8801 Thermosynechococcus elongatus Thermosynechococcus elongatus BP-1 Triticum aestivum Triticum sp. Vigna angularis Vitis vinifera Zea mays Zostera marina

Tested applications

Blue Native PAGE (BN-PAGE) Immunogold (IG) Immunolocalization (IL) Immunoprecipitation (IP) Protein extraction Western blot (WB)

Clonality

Polyclonal

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1 | PEB (4x) | protein extraction buffer

AS08 300 | Extraction buffer for quantitative isolation of total soluble/membrane protein from plant tissue and algal/bacterial cells, optimized for subsequent western blot detection in denatured conditions

123 €

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