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Anti-Hcf101 | High chlorophyll fluorescence phenotype protein
AS06 163 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana | cellular [compartment marker] of plastid stroma
- Product Info
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Immunogen: Recombinant Arabidopsis thaliana Hcf101, amino acid residues 206-532 Q6STH5, At3g24430
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 200 µl Reconstitution: For reconstitution add 200 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunolocalization (IL), Western blot (WB) Recommended dilution: 1 : 3000 (IL), 1 : 3000 (WB) Expected | apparent MW: 57 | 50.5 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Arabidopsis thaliana
Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information: Cellular [compartment marker] of plastid stroma Additional information (application): Immunolocalization has been done on isolated plastids, followed by separation into stroma and thylakoids
- Background
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Background: Hcf101 is a novel protein required for photosystem I biogenesis. Hcf101-1 is a high-chlorophyll-fluorescence hcf) Arabidopsis thaliana mutant that lacks photosystem I. In green tissue the level of this protein is stimulated by light and the protein has not been detected in roots. It has been localized in chloroplast stroma.
- Product Citations
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Selected references: Hu et al. (2017). The SUFBC2 D Complex is Required for the Biogenesis of All Major Classes of Plastid Fe-S Proteins. Plant J. 2017 Jan 19. doi: 10.1111/tpj.13483.
Bigeard et al. (2014). Proteomic and phosphoproteomic analyses of chromatin-associated proteins from Arabidopsis thaliana. Proteomics. 2014 May 31. doi: 10.1002/pmic.201400072. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts - Reviews:
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Accessories
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