Technical blog

Which isotype is a rabbit polyclonal antibody?

Read more

2024-05-06

Why bands on the blot are not distinct from each other?

Read more

2024-05-02

What parameters should be consider when transferring HMW proteins?

Read more

2024-03-01

Why some antibodies have a broad species reactivity, while the others do not?

Read more

2024-02-20

Are directly conjugated primary antibodies always the best choice?

Read more

2023-12-28

How to obtain sharper protein bands in Western blot?

Read more

2023-12-20

Are monoclonal antibodies better, compared to polyclonal antibodies?

Read more

2023-11-30

Why, to detect some proteins, 6M urea must be included in the extraction and loading buffers?

Read more

2023-09-01

I have not used this antibody before, what conditions of Western blot do you recommend?

Read more

2023-07-27

Which subclasses of immunoglobulin G are found in rabbit serum?

Read more

2023-07-18

Antibody suddenly does not work any longer, what can be the reason?

Read more

2023-07-11

Can an antibody made to a full-length protein distinguish between 2 isoforms?

Read more

2023-05-10

What is a reason for so called "ghost bands" to appear on blots?

Read more

2023-03-30

One of the ways to remove background on a Western blot

Read more

2023-03-24

How to predict in which technique a given antibody may work?

Read more

2023-03-10

Which capture antibody to use in ELISA?

Read more

2023-03-06

Why should we measure protein concentration?

Read more

2023-03-03

Can results obtained in Western blot be representative for other techniques?

Read more

2023-01-31

Effect of a membrane wash on background signal in Western blot

Read more

2022-12-16

How to check predicted species reactivity of an antibody before purchase is done?

Read more

2022-12-15

Which membrane to use for protein transfer?

Read more

2022-11-30

How to visualize a target protein in a Western blot?

Read more

2022-10-31

TBS or TBS-T, how using respective buffer for primary antibody incubation may change the outcome

Read more

2022-10-03

What are the benefits of antibodies provided in lyophilized format?

Read more

2022-10-03

What conditions to apply for detection of low abundance proteins?

Read more

2022-09-09

Can primary antibodies be re-used?

Read more

2022-09-01

How antibodies are validated - interviews with experts

Read more

2022-08-10

Which secondary, fluorescent dye conjugated antibody to use with ECFP/FITC/TRIC filter set?

Read more

2022-08-01

Advice for beginners and experienced users of Western blot technique

Read more

2022-06-20

How to save time and shorten Western blot protocol?

Read more

2022-05-16

How to transfer proteins of low molecular weight?

Read more

2022-03-21

What are the benefits of using directly conjugated primary antibodies?

Read more

2022-03-16

Antigen, immunogen and epitope, what is the difference?

Read more

2022-03-11

How to get an intense signal in Western blot using a weak antibody?

Read more

2022-02-21

How much of a specific antibody can be found in serum?

Read more

2022-01-19

What does confirmed or predicted reactivity of an antibody mean?

Read more

2021-11-26

What determines primary antibody dilution?

Read more

2021-11-15

Affinity purified antibody, what does it really mean for the end user?

Read more

2021-11-10

Can knowing the antigen sequence help identify background bands in a Western blot?

Read more

2021-10-31

Best practices for Western Blot - interviews with experts

Read more

2021-08-18

Store precious samples on a membrane instead of a freezer!

Read more

2021-06-30

Save precious time in the lab, check antigen conservation before antibody purchase

Read more

2021-05-29

Background signal and a sensitivity of ECL reagent

Read more

2021-04-30

Tubulin alpha and gamma are not suitable as a loading control for metal stress

Read more

2021-03-17

What can protein extraction buffer change in a Western blot?

Read more

2021-03-12

Antibody validation strategies - crucial to secure antibody quality

Read more

2021-02-28

What Western blot conditions to apply for high abundance proteins? 

Read more

2021-01-13

Same Western blot protocol for all antibodies?

Read more

2020-12-18

Free antibody sample, is it always the best solution?

Read more

2020-11-01

What to consider before antibody purchase?

Read more

2020-10-12