Predicted reactivityPredicted reactivity can be checked based on sequence alignment between a trarget protein from a species of confirmed and validated reactivity, and the full length protein sequence from species we intend to use an antibody for. The antigens that are used for antibody development can vary from 5 to 20 amino acid long peptides, to partial or full length proteins. Therefore, comparing the overall target protein sequence conservation between species is not relevant.What to provide us with for predicted reactivity check?
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Latest
Which isotype is a rabbit polyclonal antibody?2024-05-06 Why bands on the blot are not distinct from each other?
2024-05-02 What parameters should be consider when transferring HMW proteins?
2024-03-01 Why some antibodies have a broad species reactivity, while the others do not?
2024-02-20 Are directly conjugated primary antibodies always the best choice?
2023-12-28 How to obtain sharper protein bands in Western blot?
2023-12-20 Are monoclonal antibodies better, compared to polyclonal antibodies?
2023-11-30 Why, to detect some proteins, 6M urea must be included in the extraction and loading buffers?
2023-09-01 I have not used this antibody before, what conditions of Western blot do you recommend?
2023-07-27 Which subclasses of immunoglobulin G are found in rabbit serum?
2023-07-18
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