Effect of a membrane wash on background signal in Western blot

Why should we pay attention to membrane washes?

In the presented example, both membranes were prepared with the same set of samples, on the same day, and the whole Western blot procedure was the same except the applied membrane wash. 

Membrane A
1x15 min in TBS at room temperature
3x5 min in TBS at room temperature

Membrane B
4x5 min in TBS at room temperature

Please, pay attention to this step in your protocol!

 




 

Effect of membrane wash on background signal in Western blot

 Technical blog

Technical blog

Latest

How to remove cross-reactivity to Rubisco of a primary antibody?
2024-09-24 Which is an optimal buffer for protein extraction?
2024-07-29 Weak target protein bands and strong background at a top of a gel, what can be a reason for that?
2024-06-19 Why do some proteins always show as double bands on Western blot?
2024-05-30 How to further denature proteins transferred to PVDF membrane?
2024-05-22 Which isotype is a rabbit polyclonal antibody?
2024-05-06 Why bands on the blot are not distinct from each other?
2024-05-02 What parameters should be consider when transferring HMW proteins?
2024-03-01 Why some antibodies have a broad species reactivity, while the others do not?
2024-02-20 Are directly conjugated primary antibodies always the best choice?
2023-12-28

Archive


Close
Login / Retailer
Register
Forgot password?