Based on over 20 years of experience with troubleshooting hundreds of Western blot results, obtained on a wide range of different samples, we can share some insights for both beginners and experienced users of Western blot. How much do you know about the protein which you aim to detect? A recommended protein checklist includes:
Therefore, our advice is: study your protein through already available data first, and save your precious time in the lab! This way, your chances to succeed with the Western blot technique will considerably increase. | You are always welcome to contact Agrisera Technical Support with any antibody-related questions! |
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Which isotype is a rabbit polyclonal antibody?2024-05-06 Why bands on the blot are not distinct from each other?
2024-05-02 What parameters should be consider when transferring HMW proteins?
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2024-02-20 Are directly conjugated primary antibodies always the best choice?
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2023-11-30 Why, to detect some proteins, 6M urea must be included in the extraction and loading buffers?
2023-09-01 I have not used this antibody before, what conditions of Western blot do you recommend?
2023-07-27 Which subclasses of immunoglobulin G are found in rabbit serum?
2023-07-18
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