Protein transferPVDF membraneFrequently used membrane for protein transfer with mechanism of protein binding being hydrophobic interactions. It is robust membrane, which can be air dried (which is fixing and unfolding transferred proteins) and re-wetted and shows a good protein retention, physical strength and chemical compatibility.PVDF membrane with proteins transferred to it, can be stored at RT for up to 6 months. Described here. Nitrocellulose membraneDisplays another mechanism of protein binging, which is through hydrophobic and electrostratic interactions, which can offer an improved result for certain proteins. This membrane type is more fragile compare to PVDF, but can offer better results in some cases. |
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Latest
Which isotype is a rabbit polyclonal antibody?2024-05-06 Why bands on the blot are not distinct from each other?
2024-05-02 What parameters should be consider when transferring HMW proteins?
2024-03-01 Why some antibodies have a broad species reactivity, while the others do not?
2024-02-20 Are directly conjugated primary antibodies always the best choice?
2023-12-28 How to obtain sharper protein bands in Western blot?
2023-12-20 Are monoclonal antibodies better, compared to polyclonal antibodies?
2023-11-30 Why, to detect some proteins, 6M urea must be included in the extraction and loading buffers?
2023-09-01 I have not used this antibody before, what conditions of Western blot do you recommend?
2023-07-27 Which subclasses of immunoglobulin G are found in rabbit serum?
2023-07-18
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