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Photosynthesis Tool Kit - quantitation
This discounted antibody set contains the following high titer, well published antibodies and is suitable for Western blot using samples from a wide range of species making. This product suitable for teaching labs or mutant screening.
Benefits of using this antibody set
- Data sheet
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The kit contains following products:
AS03 037, Rabbit anti-RbcL (Form I) (50 µl)
AS10 939, Rabbit anti-PsaC (50 µl)
AS05 084, Rabbit anti-PsbA (50 µl)
AS01 017S, Rubisco protein standard (100 µl)
AS04 042S, PsaC protein standard (100 µl)
AS01 016S, PsbA protein standard (100 µl)
AS09 602-trial, Goat anti-Rabbit IgG (H&L), HRP conjugated (2 x 10 µl)- Product Info
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Immunogen: KLH-conjugated syntetic peptides for respective antibodies, see product info sheets Host: Rabbit, Secondary antibody: Goat Clonality: Polyclonal Quantity: 50 µl of respective antibody, 100 µl of each protein standard, 2 x 10 µl of secondary antibody. Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) - Reactivity
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Confirmed reactivity: Algae, Cyanobacteria, Higher plants - Additional Information
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Additional information: Product information - Primary antibodies:
Product number:
Product name:
Reconstitution:
Recommended dilution:
Rabbit Anti-RbcL
Global antibody
For reconstitution see lable on respective tube.
1:5000-10 000 with ECL
Rabbit Anti-PsaC
Global antibody
For reconstitution see lable on respective tube.
1:1000 with ECL
Rabbit Anti-PsbA
Global antibody
For reconstitution see lable on respective tube.
1:10 000 with ECL
* All primary antibodies in this kit are raised in rabbits.
Product information - Protein standards:
Product number:
Product name:
Concentration:
Size:
Western Blot – Positive Control:
PsbA *
0.25 pmol/ μl
41.5 kDa#
To generate a standard curve, 3 loads are suggested (0.5, 2 and 4 ul). For most applications a sample load of 0.2 ug of chlorophyll will give a PsbA signal in this range.
A 2 ul load is optimal for most chemiluminescent detection systems to use as a positive control.
RbcL *
0.15 pmol/ μl
52.7 kDa
To generate a standard curve, 3 loads are suggested (0.5, 2 and 4 ul). For most applications a sample load of 0.2 ug of chlorophyll will give a RbcL signal in this range.
A 2 ul load is optimal for most chemiluminescent detection systems to use as a positive control.
PsaC *
0.15 pmol/μl
11.5 kDa#
To generate a standard curve, 3 loads are suggested (0.5, 2 and 4 µl). For most applications a sample load of 0.2 µg of chlorophyll will give a PsaC signal in this range.
A 2 ul load is optimal for most chemiluminescent detection systems as a positive control.
*These proteins are larger than a respective native protein due to the addition of His-tag
* For reconstitution of standards see lable on respective tube.
Product information - Secondary antibody:
AS09 602-trial Goat anti-Rabbit IgG (H&L), HRP conjugated, 20 µl (2x10 µl)
Educational information about Quantitative western blot can be found here: detailed method description, video tutorial
Additional information (application): Estimation of PSI to PSII ratio can be done using quantitative western blot technique using anti-PsaC (PSI) and PsbA (PSII) antibodies.
References:
Brown et al. (2007). Resource dynamics during infection of Microm
onas pusilla by virus MpV-SP1. Environmental Microbiology 9(11): 2720-2727. Brown et al. (2008). Flux capacities and acclimation costs in Trichodesmium from the Gulf of Mexico. Marine Biology 154 (3): 413-422. - Product Citations
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Selected references: Abramson (2018). CARBON PARTITIONING IN ENGINEERED CYANOBACTERI UM FOR THE STUDY OF FEEDBACK INHIBITION OF PHOTOSYNTHESIS. Michigan State University, ProQuest Dissertations Publishing, 2018. 10826228.
Morash et al. (2007) Macromolecular dynamics of the photosynthetic system over a seasonal developmental progression in Spartina alterniflora. Can J. of Bot. 85: 476-483(8)
Bouchard et al. (2006) UVB effects on the photosystem II-D1 protein of phytoplankton and natural phytoplankton communities. Photochem and Photobiol 82: 936-951.
MacKenzie et al (2005). Large reallocations of carbon, nitrogen and photosynthetic reductant among phycobilisomes, photosystems and Rubisco during light acclimation in Synechococcus elongatus are constrained in cells under low environmental inorganic carbon. Arch of Microbiol. 183: 190 - 202.