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Anti-Ycf3 | Photosystem I assembly protein ycf3
AS07 273 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii, cyanobacteria
- Product Info
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Immunogen: full length recombinant ycf3 protein of Chlamydomonas reinhardtii UniProt: O20031, as described in Boudreau et al. 1997
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 200 µl Reconstitution: For reconstitution add 200 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 19 kDa
- Reactivity
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Confirmed reactivity: Chlamydomonas reinhardtii, cyanobacteria Predicted reactivity: Algae, Arabidopsis thaliana, Avena sativa, Chlorella vulgaris, Marchantia polymorpha, Phaseolus vulgaris, Physcomitrium patens, Chlorokybus atmophyticus, Ostreococcus tauri
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information (application): Western blot detection image can be found in Boudreau et al. 1997.
- Background
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Background: Ycf3 together with Ycf4 have been found as extrinistic thylakoid membrane proteins which are required for the accumulation of PSI complex in Chlamydomonas reinhardii.
- Product Citations
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Selected references: Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113. Epub 2016 Feb 22.
Naver et al. (2001). Functional studies of Ycf3. The Plant Cell 13:2731- 2746.
Boudreau et al. (1997) The chloroplast ycf3 and ycf4 open reading frames of Chlamydomonas reinhardtii are required for the accumulation of the photosystem I complex. The EMBO J.16:6095-6104. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela
Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela - Reviews:
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Accessories
AS07 274 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii, cyanobacteria
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