Agrisera reagents for successful protein detection in Western blot

Agrisera supports protein detection in your laboratory with tailored reagents with the following features:

High sensitivity
Long-term stability
Suitable for PVDF and nitrocellulose membranes
Superior lot-to-lot consistency
Competitive pricing

For chemiluminescent detection of the activity of HRP (Horseradish Peroxidase enzyme), coupled to a secondary antibody, we recommend using:
AgriseraECL Bright (AS16 ECL-N), for low pico to mid-femtogram detection (for proteins of medium and high expression), or the enhanced AgriseraECL SuperBright (AS16 ECL-S), for extreme low femtogram detection (for proteins of low expression). Both reagents are provided in one set and if one of ECL is not sensitive enough, or there is too much background, the membrane can be washed and developed again, as described here.

For laboratories without a CCD camera, visualization of the target protein can be made using so-called chromogenic detection with Agrisera BCIP/NBT ALP Substrate (AS19 BCIP-NBT) or Agrisera BCIP/NBT Plus ALP Substrate solution (AS19 BCIP-NBT-PLUS). In this case, the secondary antibody is conjugated to an enzyme called ALP (Alkaline Phosphatase). The reaction is visualized directly on the membrane through color development, and the membrane can be stored in the lab book for a future reference. This detection system is not appropriate for proteins of low expression, or to detect protein modifications, such as phosphorylation.

Agrisera reagents for protein visualization in Western blot technique

Agrisera reagents for successful protein detection in Western blot

Agrisera blog

Latest

Archive