Agrisera supports protein detection in your laboratory with tailored reagents with the following features:
For chemiluminescent detection of the activity of HRP (Horseradish Peroxidase enzyme), coupled to a secondary antibody, we recommend using: AgriseraECL Bright (AS16 ECL-N), for low pico to mid-femtogram detection (for proteins of medium and high expression), or the enhanced AgriseraECL SuperBright (AS16 ECL-S), for extreme low femtogram detection (for proteins of low expression). Both reagents are provided in one set and if one of ECL is not sensitive enough, or there is too much background, the membrane can be washed and developed again, as described here. For laboratories without a CCD camera, visualization of the target protein can be made using so-called chromogenic detection with Agrisera BCIP/NBT ALP Substrate (AS19 BCIP-NBT) or Agrisera BCIP/NBT Plus ALP Substrate solution (AS19 BCIP-NBT-PLUS). In this case, the secondary antibody is conjugated to an enzyme called ALP (Alkaline Phosphatase). The reaction is visualized directly on the membrane through color development, and the membrane can be stored in the lab book for a future reference. This detection system is not appropriate for proteins of low expression, or to detect protein modifications, such as phosphorylation. | ![]() |
Latest
Two conferences supported this week by Agrisera2024-07-13 Do not miss: 15 % off all antibodies to rice proteins
2024-07-04 Agrusera supported Young Researchers Symposium on Plant Photobiology
2024-07-02 Agrisera supported Second European Congress on Photosynthesis
2024-06-29 Do not miss: 15 % off all epitope tag antibodies until end of 2024
2024-06-28 Agrisera at Plant Biology 2024, in Honolulu, Hawaii
2024-06-27 Confirm fraction purity with Agrisera compartment marker antibodies
2024-06-22 Meet with Agrisera at Plant Biology 2024 in Honolulu
2024-06-20 Multiple Agrisera photosynthesis antibodies applied for a novel mutant characterization
2024-06-16 Agrisera Western blot workshop for South America
2024-06-14
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