Technical blog

Weak target protein bands and strong background at a top of a gel, what can be a reason for that?

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2024-06-19

Why do some proteins always show as double bands on Western blot?

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2024-05-30

How to further denature proteins transferred to PVDF membrane?

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2024-05-22

Which isotype is a rabbit polyclonal antibody?

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2024-05-06

Why bands on the blot are not distinct from each other?

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2024-05-02

What parameters should be consider when transferring HMW proteins?

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2024-03-01

Why some antibodies have a broad species reactivity, while the others do not?

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2024-02-20

Are directly conjugated primary antibodies always the best choice?

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2023-12-28

How to obtain sharper protein bands in Western blot?

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2023-12-20

Are monoclonal antibodies better, compared to polyclonal antibodies?

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2023-11-30

Why, to detect some proteins, 6M urea must be included in the extraction and loading buffers?

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2023-09-01

I have not used this antibody before, what conditions of Western blot do you recommend?

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2023-07-27

Which subclasses of immunoglobulin G are found in rabbit serum?

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2023-07-18

Antibody suddenly does not work any longer, what can be the reason?

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2023-07-11

Can an antibody made to a full-length protein distinguish between 2 isoforms?

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2023-05-10

What is a reason for so called "ghost bands" to appear on blots?

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2023-03-30

One of the ways to remove background on a Western blot

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2023-03-24

How to predict in which technique a given antibody may work?

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2023-03-10

Which capture antibody to use in ELISA?

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2023-03-06

Why should we measure protein concentration?

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2023-03-03

Can results obtained in Western blot be representative for other techniques?

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2023-01-31

Effect of a membrane wash on background signal in Western blot

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2022-12-16

How to check predicted species reactivity of an antibody before purchase is done?

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2022-12-15

Which membrane to use for protein transfer?

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2022-11-30

How to visualize a target protein in a Western blot?

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2022-10-31

TBS or TBS-T, how using respective buffer for primary antibody incubation may change the outcome

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2022-10-03

What are the benefits of antibodies provided in lyophilized format?

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2022-10-03

What conditions to apply for detection of low abundance proteins?

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2022-09-09

Can primary antibodies be re-used?

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2022-09-01

How antibodies are validated - interviews with experts

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2022-08-10

Which secondary, fluorescent dye conjugated antibody to use with ECFP/FITC/TRIC filter set?

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2022-08-01

Advice for beginners and experienced users of Western blot technique

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2022-06-20

How to save time and shorten Western blot protocol?

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2022-05-16

How to transfer proteins of low molecular weight?

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2022-03-21

What are the benefits of using directly conjugated primary antibodies?

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2022-03-16

Antigen, immunogen and epitope, what is the difference?

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2022-03-11

How to get an intense signal in Western blot using a weak antibody?

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2022-02-21

How much of a specific antibody can be found in serum?

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2022-01-19

What does confirmed or predicted reactivity of an antibody mean?

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2021-11-26

What determines primary antibody dilution?

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2021-11-15

Affinity purified antibody, what does it really mean for the end user?

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2021-11-10

Can knowing the antigen sequence help identify background bands in a Western blot?

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2021-10-31

Best practices for Western Blot - interviews with experts

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2021-08-18

Store precious samples on a membrane instead of a freezer!

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2021-06-30

Save precious time in the lab, check antigen conservation before antibody purchase

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2021-05-29

Background signal and a sensitivity of ECL reagent

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2021-04-30

Tubulin alpha and gamma are not suitable as a loading control for metal stress

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2021-03-17

What can protein extraction buffer change in a Western blot?

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2021-03-12

Antibody validation strategies - crucial to secure antibody quality

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2021-02-28

What Western blot conditions to apply for high abundance proteins? 

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2021-01-13

Same Western blot protocol for all antibodies?

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2020-12-18

Free antibody sample, is it always the best solution?

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2020-11-01

What to consider before antibody purchase?

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2020-10-12

Protein extraction, a key to success in Western blot

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2020-09-30

No signal on Western blot, what to do next?

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2020-08-31

Is sample dentaturation above 95°C, the golden standard?

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2020-07-27

Polyclonal Antibody in serum or affinity purified, what is a difference and how can it influence my results?

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2020-06-30

What are the most important factors for a successful antibody production?

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2020-06-16

Why is pre-immune serum screening of importance before antibody production starts?

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2020-06-08

Do secondary antibodies differ only in price?

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2020-06-02

Is the antibody always to blame?

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2020-05-25

TBS-T or PBS-T, does it matter which buffer is used for antibody incubations?

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2020-05-10

What is a specific antibody concentration?

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2020-05-07

FAQs about Catalog Antibodies

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2020-04-23

FAQs about Antibody Production Process

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2020-04-23

What is the safest method to store antibodies?

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2020-03-17

Is an antibody not specific or Western blot protocol is not optimized?

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2020-01-20

Common misconceptions about antibodies - II

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2019-11-25

Common misconceptions about antibodies - I

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2019-10-21

Why fractionate?

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2019-10-14

How to cite the antibodies used in scientific publications?

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2019-03-22

Save time! Use simultaneous incubation with few primary antibodies at the same time.

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2017-04-24

Which secondary antibodies to use when my protein was immunoprecipiated using an antibody?

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2017-02-16

Rapid Western blotting

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2016-05-13

How to know that a commercial antibody is going to work on a protein from my species?

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2014-02-06

Is a standarized reporting of research antibody use - a way to increase experimental reproducibility?

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2013-09-13

Agrisera protein extraction buffer and antibodies used to validate high throughput protein extraction from tough marine phytoplankton

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2010-12-02