Catalog and custom-produced Polyclonal Antibodies can be provided in different formats, such as: | 50 µg of antibody purified by affinity chromatography on Protein A or G will contain less specific antibodies than 50 µg of antibodies purified using matrix coupled with antigen (protein or peptide). For any type of conjugation with HRP, ALP or fluorophore, the antibody needs to be affinity purified on the specific antigen (used to produce it) to a specific immunoglobulin fraction. |
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Comparing antibodies between each other, why does it not work?2024-11-25 How to remove cross-reactivity to Rubisco of a primary antibody?
2024-09-24 Which is an optimal buffer for protein extraction?
2024-07-29 Weak target protein bands and strong background at a top of a gel, what can be a reason for that?
2024-06-19 Why do some proteins always show as double bands on Western blot?
2024-05-30 How to further denature proteins transferred to PVDF membrane?
2024-05-22 Which isotype is a rabbit polyclonal antibody?
2024-05-06 Why bands on the blot are not distinct from each other?
2024-05-02 What parameters should be consider when transferring HMW proteins?
2024-03-01 Why some antibodies have a broad species reactivity, while the others do not?
2024-02-20
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