This is a solution to save YOUR time. Agrisera's IncuBlocker will shorten western blot protocols by several hours. The key is that blocking, primary and secondary antibody incubations are ALL done in one step! As you see on the image to the right, the standard protocol can be shortened considerably. In this example, primary antibody incubation can be shortened to 30 minutes, which, combined with blocking and secondary antibody incubations all taking place during those 30 minutes, saves a considerable amount of time, not to mention cost. This product, called Agrisera IncuBlocker (rabbit), contains blocking reagent and a high-titer secondary anti-rabbit IgG antibody, HRP conjugated for chemiluminescent detection. It is suitable for work with primary antibodies of high titer, against medium and high abundancy proteins. IncuBlocker can be combined with Agrisera Enhanced ECL of high-sensitivity detection. | Interested to receive a free sample? Please inquire. |
Latest
Comparing antibodies between each other, why does it not work?2024-11-25 How to remove cross-reactivity to Rubisco of a primary antibody?
2024-09-24 Which is an optimal buffer for protein extraction?
2024-07-29 Weak target protein bands and strong background at a top of a gel, what can be a reason for that?
2024-06-19 Why do some proteins always show as double bands on Western blot?
2024-05-30 How to further denature proteins transferred to PVDF membrane?
2024-05-22 Which isotype is a rabbit polyclonal antibody?
2024-05-06 Why bands on the blot are not distinct from each other?
2024-05-02 What parameters should be consider when transferring HMW proteins?
2024-03-01 Why some antibodies have a broad species reactivity, while the others do not?
2024-02-20
Archive
- November - 2024
- September - 2024
- July - 2024
- June - 2024
- May - 2024
- March - 2024
- February - 2024
- December - 2023
- November - 2023
- September - 2023
- July - 2023
- May - 2023
- March - 2023
- January - 2023
- December - 2022
- November - 2022
- October - 2022
- September - 2022
- August - 2022
- June - 2022
- May - 2022
- March - 2022
- February - 2022
- January - 2022
- November - 2021
- October - 2021
- August - 2021
- June - 2021
- May - 2021
- April - 2021
- March - 2021
- February - 2021
- January - 2021
- December - 2020
- November - 2020
- October - 2020
- September - 2020
- August - 2020
- July - 2020
- June - 2020
- May - 2020
- April - 2020
- March - 2020
- January - 2020
- November - 2019
- October - 2019
- March - 2019
- April - 2017
- February - 2017
- May - 2016
- February - 2014
- September - 2013
- December - 2010