Tubulin alpha and gamma are not suitable as a loading control for metal stress

Can tubulin be used as a loading control in metal stress?

To check this, Anti-tubulin alpha chain antibodies were used on extracts from Nicotiana tabacum plants, treated with silver nanoparticles (AgNPs) with 2 different coatings.

Tubulin alpha protein content (marked by a blue arrow in the image to the right) was influenced by silver nanoparticle treatment. Similar results were obtained for tubulin gamma chain.

Therefore, it can be concluded that:

Metal induced stress affected the expression of tubulin alpha and gamma, and that therefore, these proteins cannot be used as loading controls under these conditions.




 
Western blot using anti-tubulin antibodies on metal stressed samples
All experimental details can be found here.
Courtesy of MSc, Karla Košpić, University of Zagreb, Faculty of Science Department of Biology, Croatia.
 Technical blog

Technical blog

Latest

Comparing antibodies between each other, why does it not work?
2024-11-25 How to remove cross-reactivity to Rubisco of a primary antibody?
2024-09-24 Which is an optimal buffer for protein extraction?
2024-07-29 Weak target protein bands and strong background at a top of a gel, what can be a reason for that?
2024-06-19 Why do some proteins always show as double bands on Western blot?
2024-05-30 How to further denature proteins transferred to PVDF membrane?
2024-05-22 Which isotype is a rabbit polyclonal antibody?
2024-05-06 Why bands on the blot are not distinct from each other?
2024-05-02 What parameters should be consider when transferring HMW proteins?
2024-03-01 Why some antibodies have a broad species reactivity, while the others do not?
2024-02-20

Archive


Close
Login / Retailer
Register
Forgot password?