| Western Blotting Troubleshooting Tips from experienced users of this technique were published in August 2021 on Biocompare. Interviewees for the article were: Joanna Porankiewicz-Asplund, Ph.D., Technical Support Manager at Agrisera AB Tobias Polifke, Co-founder and Managing Director at CANDOR Bioscience GmbH Rebecca Northeast, Technical Specialist at Proteintech Group. Anne Sloan, Ph.D., Technical Support Scientist at Cell Sciences Learn some tips you may not know about! |  Agrisera Western Blot Resource (protocols, videos and more) |
Technical blog
Latest
Comparing antibodies between each other, why does it not work?2024-11-25 How to remove cross-reactivity to Rubisco of a primary antibody?
2024-09-24 Which is an optimal buffer for protein extraction?
2024-07-29 Weak target protein bands and strong background at a top of a gel, what can be a reason for that?
2024-06-19 Why do some proteins always show as double bands on Western blot?
2024-05-30 How to further denature proteins transferred to PVDF membrane?
2024-05-22 Which isotype is a rabbit polyclonal antibody?
2024-05-06 Why bands on the blot are not distinct from each other?
2024-05-02 What parameters should be consider when transferring HMW proteins?
2024-03-01 Why some antibodies have a broad species reactivity, while the others do not?
2024-02-20
Archive
- November - 2024
- September - 2024
- July - 2024
- June - 2024
- May - 2024
- March - 2024
- February - 2024
- December - 2023
- November - 2023
- September - 2023
- July - 2023
- May - 2023
- March - 2023
- January - 2023
- December - 2022
- November - 2022
- October - 2022
- September - 2022
- August - 2022
- June - 2022
- May - 2022
- March - 2022
- February - 2022
- January - 2022
- November - 2021
- October - 2021
- August - 2021
- June - 2021
- May - 2021
- April - 2021
- March - 2021
- February - 2021
- January - 2021
- December - 2020
- November - 2020
- October - 2020
- September - 2020
- August - 2020
- July - 2020
- June - 2020
- May - 2020
- April - 2020
- March - 2020
- January - 2020
- November - 2019
- October - 2019
- March - 2019
- April - 2017
- February - 2017
- May - 2016
- February - 2014
- September - 2013
- December - 2010