Agrisera protein extraction buffer and antibodies used to validate high throughput protein extraction from tough marine phytoplankton

In image: comparison of ribulose-1,5-bisphosphate carboxylase oxygenase (RbcL) immunodetection from phytoplankton, after protein extraction through sonication or Precellys bead beating. RbcL was detected by immunoblotting using a chicken anti-RbcL primary antibody (AS01 017)  and a horseradish peroxidase-conjugated goat anti-chicken secondary antibody (AS09 603). MW, molecular weight protein ladder (in kDa).

The total yield of proteins extracted from each species was similar using the sonication/flash freeze protocol or Precellys® bead beating system. Using the Precellys® system, sample throughput and reproducibility are higher than with the sonication/flash freeze protocol.