Using a standard secondary antibodies, which recognize the heavy and light chain of the primary antibodyused in Western Blot application to detect immunoprecipitated samples, will always result in two bands (the heavy chain at 50 kDa and the light chain at 25 kDa). This can often interefere with target proteins we aim to visualize. Therefore:
If the protein you aim to detect in Western blot, following immunoprecipitation with an antibody, has a molecular weight of ~50 kDa, an anti-Rabbit light chain-specific antibody should be used.
If the protein of interest is ~25 kDa, an anti-rabbit IgG Fc specific antibody shoule be used, such as: Goat anti-Rabbit IgG Fc, HRP conjugated, min. cross-reactivity to bovine horse or human serum proteins.
Good luck with your research!
If the protein you aim to detect in Western blot, following immunoprecipitation with an antibody, has a molecular weight of ~50 kDa, an anti-Rabbit light chain-specific antibody should be used.
If the protein of interest is ~25 kDa, an anti-rabbit IgG Fc specific antibody shoule be used, such as: Goat anti-Rabbit IgG Fc, HRP conjugated, min. cross-reactivity to bovine horse or human serum proteins.
Good luck with your research!