Agrisera new SUMO-tag antibody

SUMO (Small Ubiquitin-like Modifier) tag is a versatile tool in proteomics, which helps in overcoming challenges associated with protein overexpression.

Using a SUMO tag offers the following advantages:

The tag can be added in the N-terminal of a target protein, which will increase protein expression and solubility, in heterologous systems like E. coli. The addition of this tag will also prevent aggregation, facilitating proper folding of the target protein.
If combined with His or GST tags, fused to the SUMO domain, it will allow two-step affinity purification.
The SUMO tag can be cleaved by SUMO-specific protease (e.g. Ulp1) with high precision, at a defined cleavage site.
The SUMO tag can be applied for proteins which are usually insoluble and expressed in low levels.
SUMOylation mimics native post-translational modification pathways, making it a tool to study SUMO-related PTMs in proteomics.
SUMO-tagged proteins are used in pull-down assays to identify binding partners.

Disadvantage: the SUMO tag is relatively large (~11 kDa), which could interfere with the protein's natural structure or function in some cases.

Western blot using anti-SUMO tag antibodies

Agrisera anti-SUMO tag antibody
Check antibody specifications here: AS23 4970

Agrisera new SUMO-tag antibody

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