Anti-Sumo tag
Product no: AS23 4970
AS23 4970 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Sumo tag
329
€
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3-6 business days
- Product Info
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Immunogen: Recombinant protein of Sumo-tag protein, expressed in E.coli. Host: Rabbit Clonality: Polyclonal Purity: Antigen affinity purified serum, in PBS pH 7.4 Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution, add 50 µl, of sterile or deionized water. Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: Depending upon fusion partner - Reactivity
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Confirmed reactivity: SUMO tag Predicted reactivity: SUMO tag
- Application Examples
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For conducted in-vitro assay, protein concentration of the purified protein was approximately 2.5 µg/well for the input in gel staining image; Proteins are expressed in Ecoli, Rosetta2 strain, and the protein origin is Arabidopsis thaliana. The sample buffer: 25mM HEPES and 100 mM NaCl and denatured using 5x Western (Protein) Loading dye to 1x, denatured at 70°C for 15 minutes. Samples were separated in the cold with 1xSDS running buffer, using BioRad mini protean apparatus with homemade 10% acryl SDS PAGE and blotted for 1h to Amersham Hybond P 0.45 µm pore size, PVDF membrane, using wet transfer (Tris-glycine buffer) with 5% MeOH at 4C, 18 Volt, overnight. Blot was blocked with 3%milk TBS-T for 1h/RT with agitation. The SUMO antibody was used at 1:2000 dilution in 1% milk TBS-T for 1h/RT with agitation. The antibody solution was decanted, and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1: 5 000 in for h/RT with agitation. The blot was washed as above and developed with a following chemiluminescent detection reagent and an image was captured after maximum 5 minutes.
Courtesy of M.Sc. Alaa Allahham, RWTH Aachen University, Germany - Background
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Background: SUMO is short for Small Ubiquitin-like Modifier, SUMO tag can be added in the N-terminal of a target protein, to increase its expression and solubility. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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