| Question: If something goes wrong with the development of a Western blot with ECL, or if I have too high background or too low signal, can I redo the reaction on the same blot? Answer: Yes! Within the same day. Simply wash the membrane with the appropriate washing buffer, used in your protocol, 1-2 times for 5-10 minutes, and apply another one of the ECL reagents from the Agrisera ECL reagent set. If there is too much background, and a very strong band for the target protein, apply Agrisera ECL Bright. If a target protein band is not detected, apply Agrisera ECL SuperBright. If you want to learn more about how to improve your Western blot, click here. |  Blot A shows an abundant photosynthetic protein, PsbA, visualized with too sensitive of a reagent, which was washed away, and the membrane was subsequently developed with less sensitive Agrisera ECL Bright (Blot B). |
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Agriseras supports and participates in conferences in 20252024-12-20 Happy holidays 2024 from Agrisera
2024-12-16 Agrisera Western blot workshop with 2B Scientific, Agrisera exclusive distributor in UK
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2024-12-13 Agrisera Western blot workshop with BiotechnoLabs, Agrisera distributor in India
2024-12-09 Agrisera Prize at Umeĺ Plant Science Centre 2024
2024-12-06 BTL Biotechno Labs Pvt. Ltd. representing Agrisera at BIOTECH 2024 conference
2024-12-05 Agrisera Western blot workshop at KBC at Umeĺ University
2024-11-25 Agrisera supported Co- and posttranslational control in chloroplasts meeting
2024-11-24 Agrisera Western blot workshop part II with Yao-Hong Biotechnology for researchers in Taiwan
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