Discover the advantages of Agrisera detection reagents for ELISA

Agrisera offers detection reagents for ELISA (Enzyme-Linked Immunosorbent Assay), a widely used laboratory technique, employed for detecting and quantifying the presence of specific proteins, antibodies, peptides, hormones, or other substances in a sample. It is a highly sensitive and specific immunological assay, based on the principles of antigen-antibody interactions.

Visualization of antibody binding is usually performed using a secondary antibody, linked to an enzyme like Alkaline Phosphatase (ALP), or Horse Radish Peroxidase (HRP). A substrate specific to the linked enzyme is then added, and visualization is achieved as described below.

Colorimetric detection: If a chromogenic substrate is used, the enzyme catalyzes a reaction that produces a colored product. The intensity of the color is proportional to the amount of the target substance in the sample. The color change is quantified using a spectrophotometer.

Chemiluminescent detection: If a chemiluminescent substrate is used, the enzyme catalyzes a reaction that produces light. The emitted light is measured using a luminometer. The advantages of chemiluminescent ELISA include its high sensitivity, wide dynamic range, and suitability for quantitative analysis. It is commonly used in various fields, including clinical diagnostics, research laboratories, and pharmaceutical development, where the accurate measurement of protein concentrations is crucial due to its versatility, sensitivity, and ability to handle numerous samples simultaneously.

Until the end of March 2024, Agrisera offers 15% discount on all detection reagents.

Use a discount code: Detect15 and order today!

Agrisera chromogenic and chemiluminescent detection reagents for ELISA offer unique sensitivity at competitive pricing, and skilled technical support to help you to set up the experiments.

Discover the advantages of Agrisera detection reagents for ELISA

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