Protein Extraction Buffer (PEB) is an extraction buffer for the disruption and solubilization of total protein from plant tissues and algal cells. The buffer contains the anionic detergent LDS, which does not form crystals while stored at 4-10°C, and is quickly ready to use. Expected yields will be 1.5-6 µg/µl total protein (recovered from standard procedure), depending on the starting material, e.g. its biological stage and homogenization method used (bead beater vs. sonication). For processing of multiple samples, simultaneous extraction using for example a bead beater is recommended, for consistency of extraction. Provided as 5 x 2 ml (4x stock), Agrisera's PEB buffer allows up to 75 isolations of plant material (using 500 μl 1x PEB for 100 mg fresh weight), or 190 isolations of algal material (using 200 μl 1x PEB for cell amounts corresponding to 4-10 μg total chlorophyll). To product page: PEB (4x) | protein extraction buffer | Chosen references: • Altuntas et al. (2020). Proline-stimulated signaling primarily targets the chlorophyll degradation pathway and photosynthesis associated processes to cope with short-term water deficit in maize. Photosynth Res. 2020 Apr;144(1):35-48. doi: 10.1007/s11120-020-00727-w. • Pérez-López et al. (2020). Transcriptome Analysis Identifies Plasmodiophora brassicae Secondary Infection Effector Candidates. J Eukaryot Microbiol. 2020 Jan 11. doi: 10.1111/jeu.12784. • Morin et al. (2019). Morin et al. (2019). Response of the sea-ice diatom Fragilariopsis cylindrus to simulated polar night darkness and return to light. Limnology and Oceanography. 9999, 2019, 1â??20. (sea-ice diatom) • Bausch, A.R., Juhl, A.R., Donaher, N.A. et al. Mar Biol (2019) 166: 80. |
Latest
Two conferences supported this week by Agrisera2024-07-13 Do not miss: 15 % off all antibodies to rice proteins
2024-07-04 Agrusera supported Young Researchers Symposium on Plant Photobiology
2024-07-02 Agrisera supported Second European Congress on Photosynthesis
2024-06-29 Do not miss: 15 % off all epitope tag antibodies until end of 2024
2024-06-28 Agrisera at Plant Biology 2024, in Honolulu, Hawaii
2024-06-27 Confirm fraction purity with Agrisera compartment marker antibodies
2024-06-22 Meet with Agrisera at Plant Biology 2024 in Honolulu
2024-06-20 Multiple Agrisera photosynthesis antibodies applied for a novel mutant characterization
2024-06-16 Agrisera Western blot workshop for South America
2024-06-14
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