With millions of antibodies offered worldwide, by hundreds of suppliers, it may be a bit overwhelming, especially for beginners, to choose the correct antibody to answer a specific questions in a research project. Here is a short checklist with what to pay attention to: Species reactivity – Has the antibody been used to detect the protein from the species we are working with? If not, a request should be sent to the supplier to confirm if the antibody may have so-called predicted reactivity to the target protein. If not, the antibody is not suitable, and false positive results can be produced. Validation data – Is validation data available on a product information sheet? For non-essential proteins, validation data should include results obtained with knockdown or knockout mutants. Western blot results showing that an antibody binds to a recombinant protein, are not relevant to the binding in a specific sample, extracted from a specific tissue or organism. Here we explain why. Recommended antibody dilution – The lower the dilution, the fewer tests can be conducted using an antibody. Here we explain why. Publication record – Being citet in scientific articles definitely confirms the performance of a given antibody. However, as there is no worldwide standard, product numbers are oftentimes not included in the "Material and Method" section. This makes it difficult to identify what antibody has been used to obtain the presented results. How to cite antibodies in scientific publication, is explained in this short video. | ![]() You are always welcome to contact us with any questions, and we will promplty help you to find the best antibody for your specific species and application. |
Latest
Two conferences supported this week by Agrisera2024-07-13 Do not miss: 15 % off all antibodies to rice proteins
2024-07-04 Agrusera supported Young Researchers Symposium on Plant Photobiology
2024-07-02 Agrisera supported Second European Congress on Photosynthesis
2024-06-29 Do not miss: 15 % off all epitope tag antibodies until end of 2024
2024-06-28 Agrisera at Plant Biology 2024, in Honolulu, Hawaii
2024-06-27 Confirm fraction purity with Agrisera compartment marker antibodies
2024-06-22 Meet with Agrisera at Plant Biology 2024 in Honolulu
2024-06-20 Multiple Agrisera photosynthesis antibodies applied for a novel mutant characterization
2024-06-16 Agrisera Western blot workshop for South America
2024-06-14
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