Quantitate NifH enzyme in samples from a wide range of organisms

Nitrogenase NifH is involved in the biological fixation of atmospheric nitrogen to ammonia. Alternative protein names are Nitrogenase component II, Nitrogenase Fe protein, Nitrogenase reductase, and FeMoCo-nitrogenase.

Agrisera Global anti-NifH antibodies are reactivie to this enzyme from the following species, as confirmed experimentally: Anabaena PCC7120, Clostridium butyricum, Codakia orbicularis, Cylindrospermopsis raciborskii CS-505, Dolichospermum sp., Nostoc sp, Rhodopseudomonas palustris, Trichodesmium sp., nodules of Trifolium repens L., Vibrio natriegens ATCC 14048

Beyond this, reactivity is predicted to the following species, based on sequence homology: Azotobacter vinelandii (Gram-), Bradyrhizobium japonicum, Cyanobacteria, Cyanothece ATCC51142, Desulfotomaculum reducens (strain MI-1), Clostridium cellobioparum, Enterobacter, genera, euryachaeotes, Klebsiella pneumonia, Magnetococcus sp., Methanobacterium thermoautotrophicum, Methanococcus maripaludis, Methylobacterium sp., Mesoorhizobium loti, Rhodopseudomonas palustris TIE-1 strain, alpha, gamma, beta proteobacteria, enterobacteria, low GC gram+, high GC gram+, able to fix atmoshperic nitrogen, Rhizobium meliloti

Confirmed and predicted reactivity is explained further here.

By using NifH antibodies, combined with NifH protein standard it is possible to quantify the enzyme using quantitative Western blot, described here, and in this video tutorial.

Until end of March 2023, both products are offered with a 20% discount.

Quantification of NifH enzyme in samples from a wide range of organisms

Total Trichodesmium sp. protein extract (lanes 6-11, 80 pmol chlorophyll loaded) extracted with PEB (AS08 300), and NifH protein standard (lanes 1-5, 0.05, 0.1, 0.3 0.75 and 1.5 pmol standard loaded)

Quantitate NifH enzyme in samples from a wide range of organisms

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