Secondary antibodies conjugated to HRP (Horseradish peroxidase) and ALP (Alkaline phosphatase) enzymes are most frequently used to visualize target proteins in Western blot, ELISA and immunolocalization. When using secondary antibodies conjugated to HRP, the detection is performed using chemiluminescence (recorded by a CCD camera or another imager), which gives high sensitivity and good resolution in both Western blot and chemiluminescent ELISA. When using antibodies conjugated to ALP, visualization of the antibody binding to the target is achieved using chromogenic reagents, and no CCD camera is necessary. Agrisera's secondary antibody collection is composed of over 220 antibodies; both highly purified and available in a basic format, suitable for Western blot, as well as adsorbed to serum from a wide range of animal species, suitable for immunolocalization and ELISA. Based on the analysis of antibody performance in available publications, conducted by CiteAB, it became very clear that Agrisera secondary antibodies are performing above market average. This means that Agrisera secondary antibodies conjugated to ALP or HRP can be used at high dilutions and are price-worthy reagents, and will provide you with a consistent performance over many experiments.
| ![]() We are always eager to provide prompt advice on which Agrisera secondary antibody is best suited for the experiments you aim to perform. Contact us at [email protected]! |
Latest
Two conferences supported this week by Agrisera2024-07-13 Do not miss: 15 % off all antibodies to rice proteins
2024-07-04 Agrusera supported Young Researchers Symposium on Plant Photobiology
2024-07-02 Agrisera supported Second European Congress on Photosynthesis
2024-06-29 Do not miss: 15 % off all epitope tag antibodies until end of 2024
2024-06-28 Agrisera at Plant Biology 2024, in Honolulu, Hawaii
2024-06-27 Confirm fraction purity with Agrisera compartment marker antibodies
2024-06-22 Meet with Agrisera at Plant Biology 2024 in Honolulu
2024-06-20 Multiple Agrisera photosynthesis antibodies applied for a novel mutant characterization
2024-06-16 Agrisera Western blot workshop for South America
2024-06-14
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