Antibodies to tags can be applied in cases when there are no dedicated antibodies against a given protein. Addition of a specific tag, like for example FLAG, His, HA, makes it possible to study target protein using specific anti-tag antibody applied in techniques like: immunofluorescence, immunoprecipiation or Western blot or purification using affinity chromatography.

The peptide sequence of a tag can be fused to N-terminus or C-terminus of a protein under investigation or inserted  within a protein. Some tags, like FLAG can be combined with other affinity tags: His, HA or myc. Historically, FLAG is a first epitope tag and the only epitope tag which is patented. 

Tag sequences are very short and recognized by high affinity antibodies, produced in wide species range: goat, chicken, mouse, rabbit. 

Some of available anti-tag antibodies are tag position sensitive and one has to take it into account, before a purchase of anti-tag antibody.



 



 Agrisera Anti-tag antibodies


Agrisera's anti-tag antibody collection can be found here.

 Agrisera blog

Agrisera blog

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