Photosynthesis Tool Kit – quantitation, PSI, PSII, Rubisco,
(Agrisera), AS04 051
Western Blot Rubisco quantitation kit
(Agrisera), AS09 409
Beside primary antibodies and quantification standards, the kits also contain matching secondary antibodies of high titer, min. 1> 25 000 1h/RT (AS09 602) and suitable chemiluminescent detection reagent, AgriseraBright in mid picogram range.
Example
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Figure 1: Immunoblot of samples of Synechococcus elongatus PCC 7942 (lanes 1-4) and Anabaena sp. PCC 7120 (lanes 5-8). Molecular weight markers (MagicMark XP, Invitrogen) are in lane 9. Recombinant PsbA protein standards are loaded in lanes 10-12 at 0.05 pmoles, 0.15 pmoles and 0.45 pmoles.
The blot was quantitated with QuantityOne software and the standard curve is shown in Figure 2.
Figure 2: Standard curve for the blot in Figure 1. Picomoles of quantitated standard is plotted on the X-axis versus the adjusted signal volume obtained on the Y-axis.
Quantitative Western Blot method is described here. You are also welcome to watch a short video tutorial.