Plant organelle/membrane isolation
- Mitochondrial fraction
- Nuclear fraction
- Plasma membrane fraction
- Thylakoid extraction
- Arabidopsis thylakoid preparation
- Arabidopsis thylakoid extraction
- Arabidopsis lumen extraction
- BBY preparation
- Chlorophyll measurements
- PSII RC extraction for cryo-EM
- Extraction of leaf proteins
- Diatom protein extraction
- Phenol protein extraction
- Protein extraction from grasses
- Ponceau membrane staining
- TCA acetone precipitation method
- Western blot protocol
- Western blot video tutorial
- Peptide neutralization/competition assay
- Simultaneous Western blot
- Quantitative Western blot
- Quantitative Western blot video tutorial
- Western blot troubleshooting
- Western blot using IgY
- Dot blot
- ELISA
- Immunoprecipitation
- Immunoprecipitation/IgY
- Immunohistochemistry
- Anti-KLH antibody removal
- Yolk delipidation
Technical information
Antibody typesPurification
- Antibody purification
- Antibody purification - small amount of protein
- IgY purification methods
- Protein purification using antibodies
- Elution of antibodies from affinity columns
Protocols > Arabidopsis thaliana Thylakoid PreparationCourtesy of Wolfgang Schröder, Umeå University.Protocol
|
0.2 M KPO₄, pH 6.5 | 50 ml (equals 10mM KPO4) | |
MgCl₂ | 1 g (equals 5 mM MgCl₂) | |
Sorbitol | 60.126 g (equals 330 mM sorbitol) | |
Millipore H₂O | 950 ml |
Resuspension buffer, 1 L
Tricine, pH 7.5 | 10 mM (stock diluted to 1 L with millipore H₂O) | |
MgCl₂ | 1 g (equals 5 mM MgCl₂) | |
Sorbitol | 9.11 g (equals 50 mM sorbitol) |
Storage buffer, 1 L
Tricine, pH 7.5 | 10 mM (stock diluted to 1 L with millipore H₂O) | |
EDTA | 1.861 g (equals 5 mM EDTA) | |
Sorbitol | 9.11 g (equals 50 mM sorbitol) |
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