Plant organelle/membrane isolation
- Mitochondrial fraction
- Nuclear fraction
- Plasma membrane fraction
- Thylakoid extraction
- Arabidopsis thylakoid preparation
- Arabidopsis thylakoid extraction
- Arabidopsis lumen extraction
- BBY preparation
- Chlorophyll measurements
- PSII RC extraction for cryo-EM
- Extraction of leaf proteins
- Diatom protein extraction
- Phenol protein extraction
- Protein extraction from grasses
- Ponceau membrane staining
- TCA acetone precipitation method
- Western blot protocol
- Western blot video tutorial
- Peptide neutralization/competition assay
- Simultaneous Western blot
- Quantitative Western blot
- Quantitative Western blot video tutorial
- Western blot troubleshooting
- Western blot using IgY
- Dot blot
- ELISA
- Immunoprecipitation
- Immunoprecipitation/IgY
- Immunohistochemistry
- Anti-KLH antibody removal
- Yolk delipidation
Technical information
Antibody typesPurification
- Antibody purification
- Antibody purification - small amount of protein
- IgY purification methods
- Protein purification using antibodies
- Elution of antibodies from affinity columns
Protocols > Lumen extraction from Arabidopsis thalianaCourtesy of Wolfgang Schröder, Umeå University.Protocol
Supernatant --> lumen Store or use directly for analysis. |
Sorbitol | 300 mM | |
Tricine-NaOH, pH 8.4 | 20 mM | |
EDTA | 10 mM |
Add just before use:
BSA | 0.25% | |
Sodium ascorbate | 4.5 mM | |
L-cysteine | 5 mM |
Resuspension buffer, 0.5 L
Sorbitol | 300 mM | |
Hepes-NaOH, pH 7.8 | 20 mM | |
MgCl₂ | 5 mM | |
EDTA | 2.5 mM | |
KCl | 10 mM |
Pyrophosphate wash buffer, 250 ml
(shock medium to break chloroplasts)
Sodium pyrophosphate, pH 7.8 | 10 mM |
Tricin buffer, 250 ml
Tricin, pH 7.8 | 2 mM | |
Sucrose | 300 mM |
Yeda press buffer, 250 ml
Sodium phosphate, pH 7.8 | 30 mM | |
MgCl₂ | 5 mM | |
NaCl | 50 mM | |
Sucrose | 100 mM | |
EDTA | 1 mM |
![](/dokument/bibliotek/Image/contact_banner_b.jpg)