Oligomer specific amyloid-beta antibodies
Discover Agrisera's unique monoclonal antibodies targeting amyloid-beta oligomers. The approach is based on a combination of high avidity and cryptic epitopes and result in a high selectivity for Abeta oligomers where oligomers are selectively detected also in the presence of stoichiometric excess of abeta monomers corresponding to more than 4 orders of magnitude.
The approach is based on a sandwich ELISA set up where the oligomer-specific antibodies are immobilized in the bottom, followed by exposure to the sample of interest. The oligomer specific antibodies will bind the oligomers with a very high binding strength, and detection of bound abeta oligomers is subsequently performed by a normal pan anti-abeta antibody. For the second step, we recommend strong binding polyclonal anti-abeta antibody or biotin labelled variants of the oligomer-specific monoclonals.
Suggested antibody pairs with excellent performance are indicated below:
Suggested sandwich ELISA pair for the detection abeta-oligomers:
ELISA setup 1
Coating OMAB, mouse monoclonal antibody (AS10 932)
Detection OMAB, Biotinylated (AS10 932B), or amyloid-beta peptide rabbit antibody (AS08 328)
ELISA setup 2
Coating: Anti-mAB-M | Mouse anti-human Abeta protein (3-10) region, oligomer-specific (clone 2D10,F6) mouse monoclonal antibodies (AS13 2716) or Anti-mAB-O | Mouse anti-human Abeta protein (3-10) region, oligomer-specific (clone 3E5,F8) mouse monoclonal antibodies (AS13 2715)
Detection: OMAB, Biotinylated (AS10 932B), or amyloid-beta peptide rabbit antibody AS08 328)
The method used to develop these antibodies is described in detail in: Brännström et al. 2014, PLoS one: A Generic Method for Design of Oligomer-Specic Antibodies.
Other antibodies (not oligomer specific) include:
Anti-Abeta (1-42) | Amyloid-beta peptide 1-42, rabbit antibodies (AS08 328)Anti-Abeta (1-42) | Amyloid-beta peptide 1-42, rabbit antibodies (AS08 357)Anti-Abeta (1-42) | Amyloid-beta peptide 1-42, chicken antibodies (AS08 329)
Other antibodies to support Alzheimer's research include:
Anti-Alpha-1-antichymotrypsin, goat antibodies (AS10 1317)
Anti-APP Delta C31 (C-terminal), rabbit antibodies (AS21 4571)
Anti-APP | Amyloid Precursor Protein (N-term), rabbit antibodies (AS20 4455)
Anti-APP | Amyloid Precursor Protein (C-term), rabbit antibodies (AS20 4456)
Anti-APP-C31 | Caspase 3 cleaved APP, C terminal fragment, rabbit antibodies (AS21 4570)
Anti-BDNF | Brain-derived neurotrophic factor, rabbit antibodies (AS13 2691)
Anti-ChAT | choline O-acetyltransferase, rabbit antibodies (AS13 2689)
Anti-GLO1 | Glyoxalase I (clone 6F10), rat antibodies (AS21 4568)
Anti-IAPP | Human IAPP (amylin) 1-37, specific for the native hormone having a disulphide-bridge between Cys2-Cys7, chicken antibodies (AS08 359)
antibodies specific for peptides with an intact disulfide bridge
Anti-PEN2 | gamma-secretase subunit PEN-2, rabbit antibodies (AS06 154)
Anti-Prealbumin | TTR transthyretin, goat polyclonal antibodies (AS10 1326)
Anti-Transthyretin 39-44, amyloid specific , mouse monolclonal antibodies (AS16 3112)
Anti-Transthyretin 56-61, amyloid specific (mouse monoclonal antibody), mouse monoclonal antibodies (AS16 3113)
Anti-VRL-1, rabbit polyclonal antibodies (AS13 2690)