Courtesy of Dr. Lea Vojta and Dr. Hrvoje Fulgosi, Laboratory for Molecular Plant Biology and Biotechnology, Division of Molecular Biology, Institute Ruđer Bošković, Croatia.
Antibody: TROL | Thylakoid rhodanese-like protein, AS19 4257.
Western blot protocol before optimization
Protein load/well: 20 µg
Blocking: 5 % milk
Primary antibody: 1: 2000 ON/4ºC
Secondary antibody: 1: 50 000 1h/RT
Western blot protocol after optimization
Protein load/well: 2 µg
Blocking: 5 % milk
Primary antibody: 1: 2000 ON/4ºC
Secondary antibody: 1: 50 000 1h/RT
Visible improvement was achieved with decreased protein load/well (10x).
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