Plant organelle/membrane isolation
- Mitochondrial fraction
- Nuclear fraction
- Plasma membrane fraction
- Thylakoid extraction
- Arabidopsis thylakoid preparation
- Arabidopsis thylakoid extraction
- Arabidopsis lumen extraction
- BBY preparation
- Chlorophyll measurements
- PSII RC extraction for cryo-EM
- Extraction of leaf proteins
- Diatom protein extraction
- Phenol protein extraction
- Protein extraction from grasses
- Ponceau membrane staining
- TCA acetone precipitation method
- Western blot protocol
- Western blot video tutorial
- Peptide neutralization/competition assay
- Simultaneous Western blot
- Quantitative Western blot
- Quantitative Western blot video tutorial
- Western blot troubleshooting
- Western blot using IgY
- Dot blot
- ELISA
- Immunoprecipitation
- Immunoprecipitation/IgY
- Immunohistochemistry
- Anti-KLH antibody removal
- Yolk delipidation
Technical information
Antibody typesPurification
- Antibody purification
- Antibody purification - small amount of protein
- IgY purification methods
- Protein purification using antibodies
- Elution of antibodies from affinity columns
Protocols > BBY preparationCourtesy of Wolfgang Schröder, Umeå University.Protocol
(For 1 kg spinach) |
Sucrose | 300 mM | |
Sodium phosphate buffer pH 7.4 | 50 mM | |
MgCl₂ | 5 mM |
Shock medium, 0.5 L
MgCl₂ | 5 mM |
Resuspension medium, 0.5 L
Sucrose | 400 mM | |
MES pH 6.5 | 50 mM | |
NaCl | 15 mM | |
MgCl₂ | 5 mM |
Wash medium, 1 L
MES pH 6.5 | 2 mM | |
MgCl₂ | 5 mM | |
NaCl | 15 mM |
20% Triton X-100 medium, 200 g
Wash medium | 160 g | |
Triton X-100 | 40 g |
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