Diatoms


Below you will find antibodies with confirmed or predicted reactivity to Diatoms.


Suggested Control Antibodies for Diatoms

AtpB | beta subunit of ATP synthase, AS05 085 | Clonality: polyclonal | Host: rabbit 
This antibody works well and detects both the chloroplastic and the mitochondrial forms of the enzyme. It usually gives a tight band and shows good reactivity. With ECL Advance detection we usually load 1 ug of total protein from diatom extracts, using this antibody.

RbcL | Rubisco large subunit, form I, AS03 037 | Clonality: polyclonal | Host: rabbit 
This antibody is very 'hot' and the RUBISCO target is abundant, so only a small load of total protein is required to get a total band. With ECL Advance we usually load 0.5 or even 0.25 ug of total protein from diatom extracts, using this antibody. So depending upon the strength of the antibody to which you are comparing, this anti-RUBISCO may be too 'hot' to be a good comparison.

PsbA | D1 protein of PSII, C-terminal, AS05 084 | Clonality: polyclonal | Host: rabbit
This antibody is used if the comparison protein is a hydrophobic membrane protein. This antibody works well but the PsbA protein is subject to fragmentation upon storage and freeze-thaw cycles. This might be a good index of the 'quality' of the total protein extracts. For this antibody we normally load 1 ug total protein, when detecting using a sensitive chemiluminescent detection system like Agrisera ECLBright (extreme low femtogram range).

Protocol for protein extraction from diatoms 



Protocols and Technical Information  •  Support  •  Western Blot Video Tutorials
 Agrisera antibodies Diatoms

 

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AS05 085-10  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: [global antibody] for plant, green alga and bacterial F-type ATP synthases

Benefits of using this antibody

180 €
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SA000003  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: higher plants and algae

This set includes ten discounted, compartment marker antibodies of your choice. Benefits of using this antibody set

Related products:
SA000001 | Plant Cell Compartment Antibody Marker Set | 3 antibodies
SA000002 | Plant Cell Compartment Antibody Marker Set | 5 antibodies

2467 €

SA000002  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: higher plants and algae

This set includes five discounted, compartment marker antibodies of your choice. Benefits of using this antibody set

Related products:
SA000001 | Plant Cell Compartment Antibody Marker Set | 3 antibodies
SA000003 | Plant Cell Compartment Antibody Marker Set | 10 antibodies

1319 €

AS08 327  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Zea mays | Cellular [compartment marker] of Golgi in immunolocalization and of COP1 in western blot

329 €
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AS08 330 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, B. rapa subsp. chinensis, C. reinhardtii, E. crus-galli, Euglena sp., H. pluvialis, N. tabacum, P. miliaceum, P. sativum, S. oleracea, Synechococcus PCC 7942, Synechocystis sp. PCC 6803, Thalassiosira guillardii, Z. mays

Benefits of using this antibody

329 €
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AS08 310  |  Clonality: Polyclonal  |  Host: Rabbit   |  Reactivity: A. thaliana, H. vulgare, C. reinhardtii, red alga Gracilaria gracilis, Medicago sativa, diatom Thalassiosira sp., P. tricornutum Bohlin, P. yunnanensis Dode, P. notoginseng, S. lycopersicum, S. tuberosum

329 €
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AS08 300  |  Extraction buffer for quantitative isolation of total soluble/membrane protein from plant tissue and algal/bacterial cells, optimized for subsequent western blot detection in denatured conditions

123 €
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AS08 282  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Diatoms

402 €
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AS07 257 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, O. sativa, Z. mays, cyanobacteria, diatoms

compartment marker of chloroplast stroma

329 €
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AS06 146  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: [global antibody] for A. thaliana, Anabaena 7120, D. brightwellii, H. vulgare, C.reinhardtii, C. zofingiensis, L. corniculatus, N. tabacum, O. sativa, P. sativum, P. vulgaris, P. tricornutum, T. pratense, S. alba, Synechococcus sp. PCC 7942, Synechocystis sp. PCC6803, T. guillardii, T. pseudonana, Triticale, U. prolifera, Z. mays

Benefits of using this antibody

329 €
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AS05 085  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: [global antibody] for plant, green alga, animal and bacterial F-type ATP synthases

Benefits of using this antibody

329 €
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AS05 084 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms | cellular [compartment marker] of thylakoid membrane

Benefits of using this antibody

329 €
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AS04 042S  |  Recombinant protein standard for quantitation

185 €
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AS04 038 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, Physcomitrella patens, algae, cyanobacteria, diatoms

Benefits of using this antibody

329 €
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AS03 037 | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: global antibody  and compartment marker for higher plants, lichens, algae, cyanobacteria, dinoflagellates, diatoms 

Benefits of using this antibody

329 €
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AS01 021A  |  Clonality: Polyclonal  |  Host: Hen  |  Reactivity: [global antibody]  for bacteria

Benefits of using this antibody 

428 €
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AS01 017S  |  Protein standard for quantitation and positive control

185 €
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AS01 017  Clonality: Polyclonal  |  Host: Hen  |  Reactivity: [global antibody] for higher plants, algae, cyanobacteria  |  cellular [compartment marker] of plastid stroma

Benefits of using this antibody

428 €
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AS01 016  |  Clonality: Polyclonal  |  Host: Hen  |  Reactivity: [global antibody] for higher plants, algae, cyanobacteria and dinoflagellates

Benefits of using this antibody

437 €
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AS03 030  |  Clonality: Polyclonal  |  Host: Chicken  |  Reactivity: [global antibody] for plant and bacterial F-type ATP synthases

Benefits of using this antibody

428 €
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