Anti-Lhc1 | from PSI of red alga

Application example

2 µg of total chlorophyll/lane of total cell extract from (1) Thalassiosira pseudonana, (2) Heterosigma akashiwo , (3) Guillardia theta, (4) Aureococcus anophagefferens, extracted with Agrisera protein extraction buffer PEB, were separated on 13-17% SDS-PAGE and blotted 2h to nitrocellulose. membranes were blocked 1h with 5% low-fat milk powder in PBS-T (0.1% TWEEN 20) and probed with anti-Lhc1 (AS08 282, 1:6000, 1h) and secondary anti-rabbit (1:5000, 1 h) antibody (HRP conjugated) in PBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in PBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescent detection reagent. Exposure time was 2 min.

Courtesy of Meriem Alami and Beverley Green, Canada

The light-harvesting system is composed of pigment-binding proteins belonging to the Lhc multigenic family. Lhc polypeptides are able to bind chlorophyll a, chlorophyll b, and xanthophyll molecules in a suitable structural and dynamic mutual arrangement, ensuring high efficiency of energy transfer processes involved in light-harvesting and photoprotection.