Diatoms


Below you will find antibodies with confirmed or predicted reactivity to Diatoms.


Suggested Control Antibodies for Diatoms

AtpB | beta subunit of ATP synthase, AS05 085 | Clonality: polyclonal | Host: rabbit 
This antibody works well and detects both the chloroplastic and the mitochondrial forms of the enzyme. It usually gives a tight band and shows good reactivity. With ECL Advance detection we usually load 1 ug of total protein from diatom extracts, using this antibody.

RbcL | Rubisco large subunit, form I, AS03 037 | Clonality: polyclonal | Host: rabbit 
This antibody is very 'hot' and the RUBISCO target is abundant, so only a small load of total protein is required to get a total band. With ECL Advance we usually load 0.5 or even 0.25 ug of total protein from diatom extracts, using this antibody. So depending upon the strength of the antibody to which you are comparing, this anti-RUBISCO may be too 'hot' to be a good comparison.

PsbA | D1 protein of PSII, C-terminal, AS05 084 | Clonality: polyclonal | Host: rabbit
This antibody is used if the comparison protein is a hydrophobic membrane protein. This antibody works well but the PsbA protein is subject to fragmentation upon storage and freeze-thaw cycles. This might be a good index of the 'quality' of the total protein extracts. For this antibody we normally load 1 ug total protein, when detecting using a sensitive chemiluminescent detection system like Agrisera ECLBright (extreme low femtogram range).

Protocol for protein extraction from diatoms 



Protocols and Technical Information  •  Support  •  Western Blot Video Tutorials
 Agrisera antibodies Diatoms

 

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AS03 037-DL650 | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: global antibody  and compartment marker for higher plants, lichens, algae, cyanobacteria, dinoflagellates, diatoms
473 €
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AS03 037-HRP Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: [global antibody] for higher plants, lichens,  algae, cyanobacteria, dinoflagellates, diatoms  |  cellular [compartment marker] of plastid stroma in higher plants and cytoplasm in cyanobacteria

Benefits of using this antibody
490 €
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AS12 2111 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, Z. mays, C. reinhardtii, S. lycopersicum

319 €
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Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items

AS08 327  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Zea mays | Cellular [compartment marker] of Golgi in immunolocalization and of COP1 in western blot

319 €
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Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items

AS14 2775 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Vigna radiata

319 €
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Buy 3 items of this product for 217.00 €/items
By Dr. Dmitry Shevela, Prof. Lars Olof Björn, Prof. Govindjee
Hardcover: 204 pages Publisher: World Scientific Publishing Company (7 Jan. 2019)
Language: English
ISBN-10: 9813223103 ISBN-13: 978-9813223103

Product Number: AS19 4290Book ; Product Dimensions: 15.2 x 1.9 x 22.9 cm
AS05 070set | Clonality: Polyclonal | Host: Rabbit | Reactivity: Dicots, monocots, algae

The set is suitable for Western blot using samples from a wide range of species making this product suitable for teaching labs or mutant screening.

Benefits of using this antibody set

1538 €

AS11 1789S  |  Protein standard for quantitation

289 €
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AS08 371S  |  Protein standard for quantitation and positive control

205 €
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Buy 2 items of this product for 154.00 €/items
Buy 3 items of this product for 132.00 €/items

AS09 458S | Protein standard for quantitation and positive control

205 €
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AS08 330S  |  Recombinant protein standard for quantitation

289 €
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AS04 051set | Clonality: Polyclonal | Host: Rabbit | Reactivity: Higher plants, algae, cyanobacteria

This discounted antibody set contains the following high titer, well published antibodies and is suitable for Western blot using samples from a wide range of species making. This product suitable for teaching labs or mutant screening.

Benefits of using this antibody set


1025 €
SA000003  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: higher plants and algae

This set includes ten discounted, compartment marker antibodies of your choice. Benefits of using this antibody set

Related products:
SA000001 | Plant Cell Compartment Antibody Marker Set | 3 antibodies
SA000002 | Plant Cell Compartment Antibody Marker Set | 5 antibodies

2395 €
SA000001  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: higher plants and algae

This set includes three discounted, well published, compartment marker antibodies of your choice. Benefits of using this antibody set

Related products:
SA000002 | Plant Cell Compartment Antibody Marker Set | 5 antibodies
SA000003 | Plant Cell Compartment Antibody Marker Set | 10 antibodies
763 €

SA000002  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: higher plants and algae

This set includes five discounted, compartment marker antibodies of your choice. Benefits of using this antibody set

Related products:
SA000001 | Plant Cell Compartment Antibody Marker Set | 3 antibodies
SA000003 | Plant Cell Compartment Antibody Marker Set | 10 antibodies

1281 €
Product no: AS18 PrimSecECL

This deal includes:
1. Primary antibody
2. Matching secondary antibody of high titer: Goat anti-rabbit, HRP conjugated (10 µl)
3. Two Agrisera chemiluminescence reagents, Agrisera ECL kit (Bright/SuperBright) trial pack
Low picogram (Agrisera Bright) and extreme low femtogram detection range (Agrisera SuperBright).
343 €

AS04 042S  |  Recombinant protein standard for quantitation

289 €
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AS09 146S  |  Recombinant protein standard for quantitation and positive control

289 €
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AS01 017S  |  Protein standard for quantitation and positive control

205 €
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AS15 2994 | Reactivity: Rubisco protein

This set enables quantification of Rubisco using ELISA in samples from a range of various species.

1019 €
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AS09 409set | Clonality: Polyclonal | Host: Rabbit

This set enables quantification of Rubisco using Western blot in samples from a range of various species  including dicots, monocots, algae, cyanobacteria. Benefits of using this antibody set
662 €

AS15 2873S |  protein standard for quantitation and positive control

205 €
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Buy 2 items of this product for 154.00 €/items
Buy 3 items of this product for 132.00 €/items
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