To secure the success of experiments with antibodies, information provided about given catalogue antibody should contain the following: 

    Host
    The host, in which an antibody of interest has been produced is crucial for a choice of a correct matching secondary antibody. 

    Clonality
    Antibodies can be poly or monoclonal or recombinant. Each of these antibody formats is offering various advantages and disadvantages. 

    Antigen
    Full length protein (purified from tissue or recombinant) or a short peptide can be used to elicit a given antibody and determines to which proteins such antibody is going to bind. The process of antigen design is shortly described here

    Species rReactivity
    Antibody reactivity to a protein from a given species, should be confirmed experimentally and can be also predicted based on sa equence conservation. 

    Applications
    Each application requires ispecific validation and product information sheet provides us with a guidance which applications has been tested so far. Recommended dilution provides a guidance for further antibody use. 

    Application examples /Validation data
    This section should contain detailed protocol describing how a given antibody was used. In case of Western blot technique, it should provide us with the information about: extraction buffer, protein load/well, mebrane transfer type and conditions, incubation buffers, blocking buffer, primary and secondary antibody dilution. 
    In case of immunolocalization, the information should contain at least type of
    tissue, fixation, blocking and primary and secondary antibody diltions. 

    References 
    For newly released antibodies, it may take 1-2 years before any publications are available. 

    Antibody validation process is explained here



      
    Things to consider:

    Host
    Most popular host for a polyclonal antibody is rabbit. Antibodies produced in different hosts, offer various advantages

    Clonality
    Polyclonal antibody is a mixture of antibodies recognizing various epitopes (from 3 to 15 amino acid long). 
    Monoclonal and recombinant antibody binds to one epitope only.

    Antigen
    If a full length recombinant protein was used to produce an antibody to a protein which comes from a family with many isoforms, an antibody may react to all isoforms. Therefore, antibodies to short peptides are called monospecific, as they target a unique sequence within a target protein, chosen following in depth bioinformatic analysis. if short peptides are used fpr antibody production, one has to inquire antibody supplier about specific peptide location to make sure that correct antibody is going to be used on the sample from species of interest. 

    Species reactivity
    Antibodies should not be used without checking that a given sequence used in its production is also found in the sequence which is aimed to be detected. This is often overlooked and leads to purchase and use of antibodies, which do not match target protein. 

    Applications
    Antibody titer in ELISA is not representative for antibody reactivity on endogenous sample and can not be used as any guarantee. 

    Application examples /Validation data
    Provided examples of antibody use, should also contain all detailes of applied protocol. If an antibody is detecting a recombinant protein, it is not a proof of its reactivity on endogenous sample, which has to be determined in a separate experiment. 

    References
    As there is no common guideline on how antibodies should be cited in scientific publications, more than a half of antibodies used in research cannot be properly identified.  
     

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