How to further denature proteins transferred to PVDF membrane?

• Following protein transfer to a PVDF membrane*, place a membrane protein side up on a Whatmann filter paper at RT.
• Let it dry for 60 minutes at RT
• Store it for further use, up to 6 months** or proceed with the next Western blot step:
• Activate PVDF in methanol, was with sterile, destilled water and place in TBS-T or PBS-T buffer for 5 minutes, before blocking.
  
• Start a blocking process in nonfat milk or other blocking agent of your choice.

* nitrocellulose membrane cannot be dried up this way

** PVDF membrane is stored in a plastic bag with a zipper or between Whatmann sheets until further use