Anti-Tubulin beta chain (monoclonal antibody)
AS20 4484 | Clonality: Monoclonal | Host: Mouse | Reactivity: Human, mouse, pig
- Data sheet
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- Product Info
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Immunogen: Beta-tubulin purified from porcine brain, UniProt: Q9H4B7 Sub class: IgM Host: Mouse Clonality: Monoclonal Purity: Purified by precipitation and affinitychromatography in TBS. Contains 15 mM sodium azide. Format: Liquid Quantity: 100 µg Storage: Store at 4°C; Do not freeze. Do not exceed exipry date is provided on the tube. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunocytochemistry (ICC), Immunohistochemistry on frozen sections (IHC), Western blot (WB) Recommended dilution: 2-8 µg/ml (ICC), 1 µg/ml (WB) Expected | apparent MW: 50.3 kDa - Reactivity
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Confirmed reactivity: Human, mouse, pig
Predicted reactivity: Plants
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information (application): This antibody is recognizing N-terminal structural domain of beta tubulin.
Recommended secondary antibody for Western blot is: goat anti mouse IgM AS16 3497
Metal induced stress affected the expression of tubulin alpha and gamma, and that therefore, these proteins cannot be used as a loading control under that type of conditions. More information can be found here. - Background
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Background: Tubulin beta (TUB) together with alpha tubulin is making up microtubules.
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts - Reviews:
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Accessories
AS10 681 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana, Medicago sativa