Diatoms

Below you will find antibodies with confirmed or predicted reactivity to Diatoms.

Suggested Control Antibodies for Diatoms

AtpB | beta subunit of ATP synthase, AS05 085 | Clonality: polyclonal | Host: rabbit
This antibody works well and detects both the chloroplastic and the mitochondrial forms of the enzyme. It usually gives a tight band and shows good reactivity. With ECL Advance detection we usually load 1 ug of total protein from diatom extracts, using this antibody.

RbcL | Rubisco large subunit, form I, AS03 037 | Clonality: polyclonal | Host: rabbit
This antibody is very 'hot' and the RUBISCO target is abundant, so only a small load of total protein is required to get a total band. With ECL Advance we usually load 0.5 or even 0.25 ug of total protein from diatom extracts, using this antibody. So depending upon the strength of the antibody to which you are comparing, this anti-RUBISCO may be too 'hot' to be a good comparison.

PsbA | D1 protein of PSII, C-terminal, AS05 084 | Clonality: polyclonal | Host: rabbit
This antibody is used if the comparison protein is a hydrophobic membrane protein. This antibody works well but the PsbA protein is subject to fragmentation upon storage and freeze-thaw cycles. This might be a good index of the 'quality' of the total protein extracts. For this antibody we normally load 1 ug total protein, when detecting using a sensitive chemiluminescent detection system like Agrisera ECLBright (extreme low femtogram range).

Protocol for protein extraction from diatoms

Protocols and Technical Information • Support • Western Blot Video Tutorials

Agrisera antibodies Diatoms

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Anti-PEPC | phosphoenolpyruvate carboxylase, HRP-conjugated (40 µg)

AS09 458-HRP | Clonality: Polyclonal | Host: Rabbit | Reactivity:A. comosus, A. thaliana, S. PCC 6803, Ch. velia, H. vulgare, J. curcas, Lupinus sp. , M. crystallinum, N. tabacum, O. sativa, Saccharum spp. hybrid clone C91-301, S. laricifolia, Z. mays, Synechocystis sp. , P. tricornutum (strain CCAP 1055/1), T. weissfloggi

Benefits of using this antibody

490 €

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Anti-PEPC | phosphoenolpyruvate carboxylase, DyLight® 650 conjugated (40 µg)

AS09 458-DL650 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. comosus, A. thaliana, C. ciliaris, C. gayana, C. velia, H. vulgare, J. curcas, K. prostrata, L. fusca, Lupinus sp. , M. maximus, M. crystallinum, N. tabacum, O. sativa, P. antidotale, P. coloratum, P. strobus, Saccharum spp. hybrid clone C91-301, S. lanata, S. laricifolia, S. bicolor, Synechocystis PCC 6803, Phaeodactylum tricornutum (strain CCAP 1055/1), T. weissfloggi, Z. mays, Z. muelleri

505 €

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Anti-PEPC | phosphoenolpyruvate carboxylase, DyLight® 594 conjugated (40 µg)

AS09 458-DL594 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. comosus, A. thaliana, C. ciliaris, C. gayana, C. velia, H. vulgare, J. curcas, K. prostrata, L. fusca, Lupinus sp. , M. maximus, M. crystallinum, N. tabacum, O. sativa, P. antidotale, P. coloratum, P. strobus, Saccharum spp. hybrid clone C91-301, S. lanata, S. laricifolia, S. bicolor, Synechocystis PCC 6803, Phaeodactylum tricornutum (strain CCAP 1055/1), T. weissfloggi, Z. mays, Z. muelleri

505 €

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Anti-PEPC | phosphoenolpyruvate carboxylase, DyLight® 488 conjugated (40 µg)

AS09 458-DL488 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. comosus, A. thaliana, C. ciliaris, C. gayana, C. velia, H. vulgare, J. curcas, K. prostrata, L. fusca, Lupinus sp. , M. maximus, M. crystallinum, N. tabacum, O. sativa, P. antidotale, P. coloratum, P. strobus, Saccharum spp. hybrid clone C91-301, S. lanata, S. laricifolia, S. bicolor, Synechocystis PCC 6803, Phaeodactylum tricornutum (strain CCAP 1055/1), T. weissfloggi, Z. mays, Z. muelleri

505 €

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Anti-PEPC | phosphoenolpyruvate carboxylase, ALP-conjugated (40 µg)

AS09 458-ALP | Clonality: Polyclonal | Host: Rabbit | Reactivity:A. comosus, A. thaliana, S. PCC 6803, Ch. velia, H. vulgare, J. curcas, Lupinus sp. , M. crystallinum, N. tabacum, O. sativa, Saccharum spp. hybrid clone C91-301, S. laricifolia, Z. mays, Synechocystis sp. , P. tricornutum (strain CCAP 1055/1), T. weissfloggi

Benefits of using this antibody

505 €

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Anti-PEPC | Phosphoenolpyruvate carboxylase

AS09 458 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. comosus, A. thaliana, C. ciliaris, C. gayana, C. velia, Ch. quinoa, H. vulgare, J. curcas, K. prostrata, L. fusca, Lupinus sp. , M. maximus, M. crystallinum, N. tabacum, O. sativa, P. antidotale, P. coloratum, P. strobus, Saccharum spp. hybrid clone C91-301, S. lanata, S. laricifolia, S. bicolor, Synechocystis PCC 6803, Phaeodactylum tricornutum (strain CCAP 1055/1), T. weissfloggi, Z. mays, Z. muelleri

Benefits of using this antibody

329 €

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Rubisco quantitation kit (Western blot)

AS09 409set | Clonality: Polyclonal | Host: Rabbit

This set enables quantification of Rubisco using Western blot in samples from a range of various species including dicots, monocots, algae, cyanobacteria. Benefits of using this antibody set

682 €

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PsbD | D2 protein of PSII positive control/quantitation standard

AS09 146S | Recombinant protein standard for quantitation and positive control

185 €

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HSP70 | Positive control/quantitation standard

AS08 371S | Protein standard for quantitation and positive control

185 €

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PetC | Rieske iron-sulfur protein of Cyt b6/f complex, protein standard

AS08 330S | Recombinant protein standard for quantitation

298 €

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Anti-PetC | Rieske iron-sulfur protein of Cyt b6/f complex

AS08 330 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, B. rapa subsp. chinensis, C. reinhardtii, E. crus-galli, Euglena sp., H. pluvialis, N. tabacum, P. miliaceum, P. sativum, S. oleracea, Synechococcus PCC 7942, Synechocystis sp. PCC 6803, Thalassiosira guillardii, Z. mays

Benefits of using this antibody

329 €

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Anti-Sec21p | Gamma subunit, COP vesicles

AS08 327 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Zea mays | Cellular [compartment marker] of Golgi in immunolocalization and of COP1 in western blot

329 €

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Anti-NR | Nitrate reductase, assimilatory

AS08 310 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, C. reinhardtii, red alga Gracilaria gracilis, Medicago sativa, diatom Thalassiosira sp., P. tricornutum Bohlin, P. yunnanensis Dode, P. notoginseng, S. lycopersicum, S. tuberosum

329 €

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1 | PEB (4x) | protein extraction buffer

AS08 300 | Extraction buffer for quantitative isolation of total soluble/membrane protein from plant tissue and algal/bacterial cells, optimized for subsequent western blot detection in denatured conditions

123 €

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Anti-Lhc1 | from PSI of red alga

AS08 282 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Diatoms

402 €

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Anti-PRK ribulose-5-P-kinase | phosphoribulokinase, Biotin-conjugated (40 µg)

AS07 257B | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, Z. mays, cyanobacteria, diatoms

compartment marker of chloroplast stroma

505 €

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Anti-PRK ribulose-5-P-kinase | Phosphoribulokinase, HRP-conjugated (40 µg)

AS07 257-HRP | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, Z. mays, cyanobacteria, diatoms

compartment marker of chloroplast stroma

505 €

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Anti-PRK ribulose-5-P-kinase | Phosphoribulokinase, DyLight® 650 conjugated (40 µg)

AS07 257-DL650 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, O. sativa, Z. mays, cyanobacteria, diatoms

compartment marker of chloroplast stroma

505 €

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Anti-PRK ribulose-5-P-kinase | Phosphoribulokinase, DyLight® 594 conjugated (40 µg)

AS07 257-DL594 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, O. sativa, Z. mays, cyanobacteria, diatoms

compartment marker of chloroplast stroma

505 €

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Anti-PRK ribulose-5-P-kinase | Phosphoribulokinase, DyLight® 488 conjugated (40 µg)

AS07 257-DL488 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, O. sativa, Z. mays, cyanobacteria, diatoms

compartment marker of chloroplast stroma

505 €

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Anti-PRK ribulose-5-P-kinase | phosphoribulokinase, ALP-conjugated (40 µg)

AS07 257-ALP | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, Z. mays, cyanobacteria, diatoms

compartment marker of chloroplast stroma

505 €

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Anti-PRK ribulose-5-P-kinase | Phosphoribulokinase

AS07 257 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, O. sativa, Z. mays, cyanobacteria, diatoms

compartment marker of chloroplast stroma

329 €

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Anti-PsbD | D2 protein of PSII

AS06 146 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for A. thaliana, Anabaena 7120, D. brightwellii, H. vulgare, C.reinhardtii, C. zofingiensis, L. corniculatus, N. tabacum, O. sativa, P. sativum, P. vulgaris, P. tricornutum, T. pratense, S. alba, Synechococcus sp. PCC 7942, Synechocystis sp. PCC6803, T. guillardii, T. pseudonana, Triticale, U. prolifera, Z. mays

Benefits of using this antibody

329 €

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Anti-AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial) (rabbit) (10 µl)

AS05 085-10 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for plant, green alga and bacterial F-type ATP synthases

Benefits of using this antibody

180 €

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Anti-AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial) (rabbit antibodies)

AS05 085 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for plant, green alga, animal and bacterial F-type ATP synthases

Benefits of using this antibody

329 €

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Anti-PsbA | D1 protein of PSII, C-terminal, Biotin-conjugated (40 µg)

AS05 084B | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms
compartment marker of thylakoid membrane

Benefits of using this antibody

505 €

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Anti-PsbA | D1 protein of PSII, C-terminal, HRP-conjugated (40 µg)

AS05 084A-HRP | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms compartment marker of thylakoid membrane

Benefits of using this antibody

481 €

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Anti-PsbA | D1 protein of PSII, C-terminal, DyLight® 650 conjugated (40 µg)

AS05 084A-DL650 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms compartment marker of thylakoid membrane

505 €

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Anti-PsbA | D1 protein of PSII, C-terminal, DyLight® 594 conjugated (40 µg)

AS05 084A-DL594 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms compartment marker of thylakoid membrane

505 €

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Anti-PsbA | D1 protein of PSII, C-terminal, DyLight® 488 conjugated (40 µg)

AS05 084A-DL488 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms compartment marker of thylakoid membrane

505 €

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