Anti-PIP (PIP1;1, PIP1;2, PIP1;3, PIP1;4, PIP1;5) aquaporins, DyLight® 650 conjugated (40 µg)

Product no: AS09 487-DL650
AS09 487-DL650 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, J. curcas, P. nigra, P. trichocarpa, R. sativus
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  • Product Info
  • Immunogen: KLH-conjugated synthetic peptide conserved in Arabidopsis thaliana: PIP1;1 UniProt: P61837,At3g61430 PIP1;2 UniProt: Q06611,TAIR: At2g45960 PIP1;3 UniProt: Q08733,TAIR: At1g01620 , PIP1;4 UniProt: Q39196,TAIR: At4g00430, PIP1;5 UniProt: Q8LAA6 TAIR:At4g23400
    Host: Rabbit
    Clonality: Polyclonal
    Purity:

    Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight® 650.

    Format: Liquid in PBS pH 7.4.
    Quantity: 40 µg
    Storage: Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years. Spin briefly the tube before use.
    Tested applications: Immunofluorescence (IF), Western blot (WB)
    Recommended dilution: To be determined by end user.
    Expected | apparent MW: 30.68 | 28 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Brassica sp,, Jatropha curcas L, cv, Biji Jarak , Mesembryantheum crystallinum, Populus nigra, Populus trichocarpa, Raphanus sativus, Thellungiella salsuginea
    Predicted reactivity: Brassica sp., Hordeum vulgare, Juglans regia, Nicotiana tabacum, Oryza sativa, Populus tremula, Triticum aestivum, Vicia faba

    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known.
  • Additional Information
  • Additional information: Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

    DyLight® 650 has Amax = 652 nm, Emax = 672 nm. DyLight® is a registered trademark of Thermofisher Inc., and its subsidiaries.
    Additional information (application): Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
  • Background
  • Background: PIPs proteins are aquaporins which facilitate the transport of water and small neutral molecules across the cell membrane. Alternative names: PIP1-1, PIP1-2, PIP1-3, plasma membrane intrinsic protein 1a, 1b, 1c, PIP1a, PIP1b, PIP1c, plasma membrane aquaporin-1, transmembrane protein A, TMP-A, AthH2, transmembrane protein B, TMP-B.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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