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Anti-TIP2 | Tonoplast intrinsic protein 2;1, 2;2, 2;3
AS12 2619 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
- Product Info
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Immunogen: KLH-conjugated synthetic peptide conserved in Arabidopsis thaliana TIP2;1 (100 %) UniProt:Q41951, TAIR:AT3G16240, TIP2;2 (90%) UniProt: Q41975, TAIR:AT4G17340, TIP2;3 (81%) UniProt: Q9FGL2, TAIR: AT5G47450
Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4. Format: Lyophilized Quantity: 200 µg Reconstitution: For reconstitution add 200 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunolocalization (IL), Western blot (WB) Recommended dilution: 1:100 (IL), 1 : 1000 -1 : 2000 (WB) Expected | apparent MW: 25.6 | 24 kDa (Arabidopsis thaliana)
- Reactivity
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Confirmed reactivity: Arabidopsis thaliana, Quercus suber Predicted reactivity: Antirrhinum majus, Brassica oleracea, Chorispora exscapa, Gossypium mexicanum, Helianthus annuus, Medicago truncatula, Malus prunifolia, Nicotiana tabacum, Populus balsamifera subsp. trichocarpa, Raphanus sativus, Ricinus communis, Solanum nigrum , Solanum tuberosum, Vitis vinifera, Quercus petraea
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Application example 
10 µg of total proteinfrom Arabidopsis thaliana roots (Ecotype-Columbia) were separated on 12%SDS-PAGE and blotted 1h to PVDF membrane. Blots were blocked with 5%low-fat milk powderfor 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 over night with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T (0.05% Triton X.100)at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidaseconjugated from donkey diluted to 1:15 000 in for 1h at RT with agitation. The blot was washed as above and developed with chemiluminescent reagent, according to the manufacturer's instructions. Exposure time was 5 seconds.
Courtesy of Dr. Azeez Suliman Beebo, University of Gothenburg, Sweden - Additional Information
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Additional information (application): Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel - Background
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Background: TIP2 (Aquaporin TIP) is a protein required for the transport of water from the vacuolar compartment to the cytoplasm. Alternative names: Delta-tonoplast intrinsic protein, Delta-TIP.
- Protocols
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Agrisera Western Blot protocol and video tutorials
Method for isolation of plant vacuolar membranes
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Courtesy of Dr. Masayoshi Maeshima, Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences Nagoya University Nagoya, Japan
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