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Anti-DYKDDDDK (binds to Sigma FLAG®) (mouse monoclonal) (clone F-tag-01)
- Product Info
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Sub class: IgG1 Immunogen: KLH-conjugated synthetic peptide: DYKDDDDK (Sigma FLAG®) Host: Mouse Clonality: Monoclonal Purity: Immunoglobulin purified on Protein A in PBS pH 7.4. Contains 15 mM sodium azide. Format: Liquid at 1 mg/ml. Quantity: 0.1 mg Storage: Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunocyto chemistry (ICC), Western blot (WB) Recommended dilution: 1 µg/ml (WB) - Reactivity
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Confirmed reactivity: DYKDDDDK (Sigma FLAG®) epitope tag - Application Examples
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COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DYKDDDDK (binds to Sigma FLAG®.
A - fusion nuclear protein (red) stained with anti-DYKDDDDK (binds to Sigma FLAG® monoclonal antibodies at 1 µg/ml, secondary antibody goat anti-mouse IgG1 Alexa Fluor® 594
B - cell nuclei stained with DAPI (blue)
C - merged figures - confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue) - Additional Information
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Additional information: This antibody recognizes DYKDDDDK (Sigma FLAG®) tagged proteins - Background
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Background: Mouse anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (Sigma FLAG®). The small size of this tag and its high hydrophilicity decrease the probability of interference with its expression, proteolytic maturation, antigenicity, localization and function. - Protocols
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Agrisera Western Blot protocol and video tutorials
Antibody Protocols - Reviews:
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Accessories
AS09 627 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Mouse IgG (H&L)
This product can be purchased in 3 different volumes:
AS16 ECL-S-10, 10 ml. Trial size limited to one per customer
AS16 ECL-S-100, 100 ml
Choose the appropriate volume in the drop down menu to the right
This product can be purchased in 3 different volumes:
AS16 ECL-N-10, 10 ml. Trial size limited to one per customer
AS16 ECL-N-100, 100 ml
Choose the appropriate volume in the drop down menu to the right
AS15 3037 | Clonality: Polyclonal | Host: Rabbit | Reactivity: DYKDDDDK-tag
Replaced by AS20 4442