Anti-DYKDDDDK (binds to Sigma FLAG®) (rabbit antibody, polyclonal)
Product no: AS15 3037
AS15 3037 | Clonality: Polyclonal | Host: Rabbit | Reactivity: DYKDDDDK-tag
Replaced by AS20 4442
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- Product Info
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Immunogen: KLH-conjugated synthetic peptide: DYKDDDDK (Sigma FLAG®).
Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified in PBS, pH 7.4. Contains 0.055% sodium azide. Format: Liquid Quantity: 100 µg Storage: Store at -20°C for up to 1 year, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB) Recommended dilution: 1 : 1000 (WB) 1 : 200 (IP), 1 : 200 (IHC) - Reactivity
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Confirmed reactivity: DYKDDDDK epitope tag (Sigma FLAG®), fused to proteins in plant and mammalian cells Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Background
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Background: Rabbit anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (Sigma FLAG®).
- Product Citations
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Selected references: Salesse-Smith et al. (2018). Overexpression of Rubisco subunits with RAF1 increases Rubisco content in maize. Nat Plants. 2018 Oct;4(10):802-810. doi: 10.1038/s41477-018-0252-4. - Protocols
- Agrisera Western Blot protocol and video tutorials
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