Anti-DYKDDDDK (binds to Sigma FLAG®), HRP conjugated (Clone M2)
- Product Info
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Immunogen: DYKDDDDK (Sigma FLAG®)
Host: Mouse Clonality: Monoclonal Purity: Protein A purified. Format: Lyophilized Quantity: 100 µg Reconstitution: For reconstitution add 100 µl of sterile water Storage: Store freeze-dried poweder at 2-8°C.Rehydrate with 1.0 ml of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily. Shelf life: 1 year from date of receipt. Prepare working dilution prior to use and then discard. Tested applications: Western blot (WB) Recommended dilution: 100 ng/ml (ELISA), 1 : 20-1 : 50 (ICC), 1 : 20-1 : 50 (IF), 3 µg (IP), 1 : 10-1 : 50 (WB) - Additional Information
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Additional information: Antibody is provided in: 100 mM NaPO4 (pH 7,4), 100 mM NaCl, 1,5% BSA, 50 mM Sucrose, 0,01 % Thimerosal,Affinity purified antibody has over 95% purity based on SDS-PAGE - Background
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Background: Mouse anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (Sigma FLAG®) and is directly conjugated to HRP, Horseradish Peroxidase. This is of advantage to shorten assay time by no need to use a secondary antibody to DYKDDDDK. - Reviews:
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Accessories
AS15 2871 | Clonality: monoclonal | Host: Mouse | Reactivity: N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins
AS15 3037 | Clonality: Polyclonal | Host: Rabbit | Reactivity: DYKDDDDK-tag
Replaced by AS20 4442