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Anti-PIN2 | Auxin efflux carrier component 2 (monoclonal)
AS21 4697 | Clonality: Monoclonal | Host: Mouse | Reactivity: Zea mays
- Product Info
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Immunogen: KLH-conjugated mixture of two synthetic peptides derived from Arabidopsis thaliana PIN2 protein sequence, UniProt: Q9LU77, TAIR:At5g57090 Host: Mouse Clonality: Monoclonal Purity: Antigen affinity purified serum, in PBS pH 7,4 Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution, add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes Tested applications: Immunolocalization (IL) Recommended dilution: 1 : 200 (IL) Expected | apparent MW: 69.3 kDa - Reactivity
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Confirmed reactivity: Zea mays Predicted reactivity: Arabidopsis thaliana, Beta vulgaris, Brassica napus, Camelina sativa, Cannabis sativa, Capsella rubella, Cucumis melo, Eucalyptus grandis, Eutrema salsugineum, Glycine max, Malus domestica, Morus notabilis, Prunus dulcis, Raphanus sativus, Spinacia oleracea, Vitis vinifera
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Type of material: Maize coleoptile stage embryos Fixation: formaldehyde | Methanol, 37°C. Both FFA and Methanol, sequentially. Hydrophilization: yes Cell wall digestion: yes Membrane permeabilization: Proteinase K incubation Antigen retrieval: no Blocking buffer: 3% BSA in 1x PBS Washing buffer: 1x PBS-T Primary antibody dilution and incubation time: 1% BSA in 1x PBS, 1:50, overnight at 4°C. Secondary antibody: 1% BSA in 1x PBS, 1:50, overnight at 4°C, Alexa fluor-488 (Anti-Rabbit and -Mouse, Thermo Fisher) Co-staining of the nucleus (DAPI): no Cell wall and nucleus staining: Calcofluor white
- Background
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Background: Arabidopsis thaliana auxin efflux carrier component AtPIN2 encoded by the AtPIN2 gene (also known as EIR1 and AGR1). AtPIN proteins are asymmetrically localized within plant plasma membranes and mediate polar auxin transport. AtPIN2 is a key regulator of the response of Arabidopsis roots to gravity. Alternative names: Auxin efflux carrier AGR, Ethylene-insensitive root 1, AtEIR1, Polar-auxin-transport efflux component AGR1, Protein AGRAVITROPIC 1, AtAGR1, Protein WAVY 6. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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Accessories
AS07 260 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for di- and monocots, conifers, ferns, mosses, green algae | Cellular [compartment marker] for plasma membrane
Benefits of using this antibody
AS22 4801 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Zea mays
AS16 3957 | Clonality: Polyclonal | Host: Goat | Reactivity: Arabidopsis thaliana