Anti-PIN1 | Auxin efflux carrier component 1 (monocots)
AS22 4801 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Zea mays
- Product Info
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Immunogen: KLH-conjugated peptide derived from Zea mays PIN1, UniProt: Q0MWY2 Host: Rabbit Clonality: Polyclonal Purity: Antigen affinity purified serum, in PBS pH 7.4 Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution, add 50 µl, of sterile or deionized water. Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications: Immunofluorescence (IF) Recommended dilution: 1 : 50 (IF) Expected | apparent MW: 65.194 | kDa (due to N-terminal or C-terminal processing) - Reactivity
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Confirmed reactivity: Zea mays Predicted reactivity: Oryza sativa
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Type of material: Maize coleoptile stage embryos
Fixation: formaldehyde | Methanol, 37°C. Both FFA and Methanol, sequentially.
Hydrophilization: yes
Cell wall digestion: yes
Membrane permeabilization: Proteinase K incubation
Antigen retrieval: no
Blocking buffer: 3% BSA in 1x PBS
Washing buffer: 1x PBS-T
Primary antibody dilution and incubation time: 1% BSA in 1x PBS, 1:50, overnight at 4°C.
Secondary antibody: 1% BSA in 1x PBS, 1:50, overnight at 4°C, Alexa fluor-488 (Anti-Rabbit and -Mouse, Thermo Fisher)
Co-staining of the nucleus (DAPI): no
Cell wall and nucleus staining: Calcofluor white - Background
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Background: PIN1 (Auxin efflux carrier component 1) is a membrane protein which may act as a component of the auxin efflux carrier. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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