Anti-PIN1 | Auxin efflux carrier component 1 (monocots)

Product no: AS22 4801

AS22 4801  | Clonality:  Polyclonal |  Host:  Rabbit | Reactivity: Zea mays

Delivery:  Under development
  • Product Info
  • Immunogen: KLH-conjugated peptide derived from Zea mays PIN1, UniProt: Q0MWY2
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Antigen affinity purified serum, in PBS pH 7.4
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution, add 50  µl, of sterile or deionized water.
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Tested applications: Immunofluorescence (IF)
    Recommended dilution: 1 : 50 (IF)
    Expected | apparent MW: 65.194 | kDa (due to N-terminal or C-terminal processing)
  • Reactivity
  • Confirmed reactivity: Zea mays
    Predicted reactivity: Oryza sativa

    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Immunofluorescence using anti-PIN1 antibodies (monocotyl)

    Type of material: Maize coleoptile stage embryos
    Fixation: formaldehyde | Methanol, 37°C. Both FFA and Methanol, sequentially.
    Hydrophilization: yes
    Cell wall digestion: yes
    Membrane permeabilization: Proteinase K incubation
    Antigen retrieval: no
    Blocking buffer: 3% BSA in 1x PBS
    Washing buffer: 1x PBS-T
    Primary antibody dilution and incubation time: 1% BSA in 1x PBS, 1:50, overnight at 4°C.
    Secondary antibody: 1% BSA in 1x PBS, 1:50, overnight at 4°C, Alexa fluor-488 (Anti-Rabbit and -Mouse, Thermo Fisher)
    Co-staining of the nucleus (DAPI): no
    Cell wall and nucleus staining: Calcofluor white
  • Background
  • Background: PIN1 (Auxin efflux carrier component 1) is a membrane protein which may act as a component of the auxin efflux carrier.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
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