Anti-PIN1 | Auxin efflux carrier component 1 (monocots)

Name: Anti-PIN1 | Auxin efflux carrier component 1 (monocots)
SKU: AS22 4801
Availability: OutOfStock

Product no: AS22 4801

AS22 4801 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Zea mays

DATA SHEET IN PDF

Delivery: Under development

Product Info

Immunogen:	KLH-conjugated peptide derived from Zea mays PIN1, UniProt: Q0MWY2
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Antigen affinity purified serum, in PBS pH 7.4
Format:	Lyophilized
Quantity:	50 µg
Reconstitution:	For reconstitution, add 50 µl, of sterile or deionized water.
Storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications:	Immunofluorescence (IF)
Recommended dilution:	1 : 50 (IF)
Expected \| apparent MW:	65.194 \| kDa (due to N-terminal or C-terminal processing)

Reactivity

Confirmed reactivity:	Zea mays
Predicted reactivity:	Oryza sativa Species of your interest not listed? Contact us
Not reactive in:	No confirmed exceptions from predicted reactivity are currently known

Application Examples

Immunofluorescence using anti-PIN1 antibodies (monocotyl)

Type of material: Maize coleoptile stage embryos
Fixation: formaldehyde | Methanol, 37°C. Both FFA and Methanol, sequentially.
Hydrophilization: yes
Cell wall digestion: yes
Membrane permeabilization: Proteinase K incubation
Antigen retrieval: no
Blocking buffer: 3% BSA in 1x PBS
Washing buffer: 1x PBS-T
Primary antibody dilution and incubation time: 1% BSA in 1x PBS, 1:50, overnight at 4°C.
Secondary antibody: 1% BSA in 1x PBS, 1:50, overnight at 4°C, Alexa fluor-488 (Anti-Rabbit and -Mouse, Thermo Fisher)
Co-staining of the nucleus (DAPI): no
Cell wall and nucleus staining: Calcofluor white

Background
Background: PIN1 (Auxin efflux carrier component 1) is a membrane protein which may act as a component of the auxin efflux carrier.
Protocols
Agrisera Western Blot protocol and video tutorials

Protocols to work with plant and algal protein extracts

Agrisera Educational Poster Collection
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